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Analysis of Polysaccharides Compositions in Ripe Peel of Citrus reticulate ’Chachi’

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【Author】 CHEN Si1,3,HUANG Qing-hua1*,YOU Ming-xia2,WU Shao-hui1,LI Rao-ling1,GAN Wei-fa1 1.Guangdong Pharmaceutical University,Guangzhou 510006,China;2.Guangdong By-Health Biotechnology Co.,Ltd.,Zhuhai 519040,China;3.Joincare Pharmaceutical Group Industry Co.,Ltd.,Shenzhen 518057,China

【摘要】 [Objective] To study the polysaccharides compositions in ripe peel of Citrus reticulate ’Chachi’.[Method] Employing HPLC method,PMP pre-column derivatization was performed to measure the monosaccharide composition of pectic polysaccharides of Citrus reticulate ’Chachi’,under the chromatographic conditions as follows:chromatographic columns of the Phenomenex Luna C18(250 mm×4.60 mm,5 μm),with the mobile phases of 0.05 mol/L mixture solution of phosphates-acetonitrile(80:20,V/V,pH=7.1),flow rate of 1.0ml/min;detection wavelength of 245 nm,column temperature of 30 ℃ and injection volume of 20 μl.[Result] The pectic polysaccharides of Citrus reticulate ’Chachi’ contained mannose,ribose,rhamnose,galacturonic acid,glucose,galactose,xylose,arabinose and so on.The mole ratios of each monosacchavide was correspondingly 0.08:0.002:0.05:4.80:1:1.52:0.11:3.82.[Conclusion] This method has the advantages of simple operation,shortened period,repeatability and yield,and could be used for the measurement of polysaccharides of Citrus reticulate ’Chachi’.

【基金】 Trade and Information Technology Commission of Zhuhai City(2009414);Student Innovation Program form Department of Education,Guangdong Province(1057310008)
  • 【分类号】S666
  • 【被引频次】1
  • 【下载频次】15
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