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Determination of Resveratrol Content in RHIZOMA ET RADIX POLYGONI CUSPIDATI by HPLC

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【Author】 XIE Bing* College of Chemistry and Environmental Science,Guizhou University for Nationalities,Guiyang,Guizhou 550025,China

【摘要】 [Objective] Based on optimizing a variety of experimental conditions,a method for the determination of resveratrol in RHIZOMA ET RADIX POLYGONI CUSPIDATI by HPLC was established by using ethanol as extractant.[Method] Phenomenex C18(250 mm×4.6 mm,5 μm) was selected as column,with methanol-0.25% acetic acid(V/V,65:35) as mobile phase,0.8 ml/min mobile speed and 306 nm detection wavelength.Taking the extraction rate of resveratrol as the evaluation index,single factor test and L25(54) orthogonal test were used to optimize the experimental conditions.[Result] The extraction conditions for resveratrol were 60% ethanol,1:15(g/ml) solid-liquid ratio,55 ℃ extraction temperature and 15 min extraction time.Under these conditions,the extraction rate of resveratrol reached 0.826 μg/g.[Conclusion] This method is proved to be simple,rapid,accurate and could be used for the determination of resveratrol content in RHIZOMA ET RADIX POLYGONI CUSPIDATI.

【基金】 Supported by the Project of State Ethnic Affairs Commission(09GZ07);Key Project of Education Department of Guizhou Province(2007051)
  • 【分类号】R284
  • 【下载频次】22
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