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Analysis of the Flavonoids in the Leaves of Rhododendron pulchrum Sweet by HPLC-MS

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【Author】 ZHANG Mei1,2,PAN Da-ren2,ZHOU Yi-fei3,ZHU Qiu-qiang2,WANG Shao-ming2 1.Minjiang University,Fuzhou,Fujian 350108,China;2.College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;3.College of Crop Sciences,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China

【摘要】 [Objective] To investigate the flavonoids in the leaves of Rhododendron pulchrum sweet by high performance liquid chromatograph - electrospray ionization tandem mass spectrometry. [Method]The chromatographic separation was performed on a C18column ( 4. 6 mm × 250 mm,5 μm) . The mobile phase A waswater containing 0. 1% formic acid and mobile phase B was anhydrous methanol. Gradient eluted program was: 0 - 24 min,31% - 42% B; 24 - 30 min,42% -50% B; 30 - 35 min,50% - 60% B; 35 - 40 min,60% B. Injection volume was 20 μl; flow rate was 0. 7 ml / min; column temperature was 30 ℃ ; and detectionwavelength was 356 nm. Mass spectrometer conditions were ESI,anion mode scanning,drying gas temperature 350 ℃ ,drying gas volume flow ( N2) 10 L/min,nebulizing gas pressure 275. 8 KPa,capillary voltage 3. 5 kV,capillary export voltage 100 V,and mass scan range m / z 200 - 700. Data were collected in anionmode. [Result] Compared with the standard references and literature data,five flavonoids compounds were identified as hyperoside,isoquercitrin,avicularin,quercitroside and quercetin. [Conclusion]This method was rapid,accurate and effective which could be applied for the constituent identification of R. pulchrum,and provided theoretical basis for the application of R. pulchrum.

【基金】 Supported by the Natural Science Foundation of Fujian Province(2008J0041)
  • 【分类号】R284.1
  • 【被引频次】3
  • 【下载频次】30
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