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Determination of Puerarin and Paeoniflorin in Yanbao I Capsule by HPLC

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【Author】 TAN Jia-hua,LUO Jun ,HE Zhu-ying,ZHANG Jian-ling,YU Jia The First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine,Guiyang,Guizhou 550001,China

【摘要】 [Objective]To establish the method for determining puerarin and paeoniflorin in Yanbao I capsule.[Method]The HPLC analysis used chromatography glass column Diamonsil C18(2)(4.6 mm×250 mm,5μm)with the detection wavelength at 250 nm for puerarin and 230 nm for paeoniflorin and the column temperature was set at 25℃.Following the injection of sample solution,an elution was initiated at a flow rate of 1 ml/min with anhydrous methanol-water(V/V,28∶72)for puerarin and acetonitrile-0.1%phosphoric acid(V/V,14∶86)for paeoniflorin as mobile phase.[Result]The linear range of puerarin was from 0.001 18 to 0.007 08μg/ml(r=0.999 9).The average recovery of sample was 102.4%with RSD of 1.17%(n=6).The linear range of paeoniflorin was from 0.044 to 0.261μg/ml(r=0.999 9).The average recovery of sample was 97.8%with RSD of 1.71%(n=6).In Yanbao I capsule,the content of puerarin and paeoniflorin could reach 0.055 and 0.168 mg/g,respectively.[Conclusion]The method is simple,accurate and repeatable,which is suitable for determining the content of puerarin and paeoniflorin in Yanbao I capsule.

【基金】 Supported by the Science and Technology Research Projects for Traditional Chinese Medicine;Folk Medicine of Guizhou Administration of Traditional Chinese Medicine(QZYY2010-28)
  • 【分类号】R286.0
  • 【被引频次】2
  • 【下载频次】46
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