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Determination of the Diosgenin Content of Dioscorea opposita Thunb. Tubers and Bulbils

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【作者】 TENG Jing-tongGAO XiangXUE Jian-pingLI Zi-weiZHANG Ai-minSHENG Wei

【Author】 TENG Jing-tong, GAO Xiang, XUE Jian-ping, LI Zi-wei, ZHANG Ai-min, SHENG Wei School of Life Science, Huaibei Normal University, Huaibei, Anhui 235000, China

【机构】 School of Life Science, Huaibei Normal University

【摘要】 [Objective] To determine the diosgenin content of Dioscorea opposita Thunb. tubers and bulbils. [Method] With 5% vanillin-glacial acetic acid-perchloric acid as color developing agent and oleanolic acid as the control substance, the content of diosgenin in D. opposita tubers and bulbils was measured using an ultraviolet visible spectrophotometer at the detection wavelength of 547nm. [Result] The content of diosgenin in D. opposita tubers and bulbils was 0.0164% and 0.0213%, respectively. The average recovery of diosgenin was 98.46% with relative standard deviations (RSD) of 1.36%. Besides, the content of diosgenin in bulbil was slightly higher than that in D. opposita tubers. [Conclusion] The method is simple, easy, accurate, and suitable for content determination of diosgenin in D. opposita tubers and bulbils.

【基金】 Supported by the Project of Anhui Key Laboratory of Plant Resources and Biology
  • 【分类号】R284.1
  • 【被引频次】2
  • 【下载频次】41
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