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Tissue Culture of the Shoot Tips of Bozhou Paeonia lactiflora Pall

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【作者】 MA Zong-xinMA Tong-fuMENG Dong-qing

【Author】 MA Zong-xin1, MA Tong-fu2, MENG Dong-qing2 1. Fuyang Institute of Agricultural Sciences, Fuyang, Anhui 236065, China; 2. Fuyang Teachers College, Fuyang, Anhui 236041, China

【机构】 Fuyang Institute of Agricultural SciencesFuyang Teachers College

【摘要】 [Objective] To study the effects of culture conditions on the tissue culture of shoot tips of Bozhou Paeonia lactiflora Pall. [Method] Culture mediums with different concentration combinations of 6-BA, NAA and IBA were prepared. Based on the cultivation results, the optimal formula was selected. [Result] After shoot tips of Bozhou P. lactiflora were cultivated on 1/2 MS+ 1.0mg/L 6-BA for 39d, buds and multiple shoots were differentiated. Multiple shoots showed the rapidest proliferation on 1/2 MS+ 1.0mg/L6-BA, and the optimal rooting results on 1/2 MS+0.1mg/L IBA. [Conclusion] Concentration of plant growth regulator directly affected the tissue culture of shoot tips of Bozhou P. lactiflora. And shoot tips could directly grow into seedlings under appropriate culture medium.

  • 【分类号】S567.239
  • 【下载频次】63
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