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Extraction and Determination of Polysaccharides from FRUCTUS CHAENOMELIS

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【作者】 LI Shi-gangLI FangTANG Yong-liXIANG Zheng-zongCHEN Yan

【Author】 LI Shi-gang, LI Fang, TANG Yong-li, XIANG Zheng-zong, CHEN Yan Medical College, China Three Gorges University, Yichang, Hubei 443002,China

【机构】 Medical College, China Three Gorges University

【摘要】 [Objective] To establish a method for the extraction and determination of polysaccharides from FRUCTUS CHAENOMELIS. [Methods] Ethanol reflux method was adopted to extract polysaccharides from FRUCTUS CHAENOMELIS. Then, the content of polysaccharides was determined by the method of C6H5OH phenol-H2SO4. [Results] The linear regression equation was Y=0.008 8X-0.010 9, R2=0.992 3. There was good linearity between concentration and absorbance of polysaccharides in the range of 1.0 to 5.0 μg/ml. Through twice extraction, the content of polysaccharides was 13.21% in defatted sample and 9.26% in original sample. The average recovery was 97.04% with RSD of 3.52%. [Conclusion] This method is simple, stable, precise and reproducible, which is suitable for extraction and determination of polysaccharides from FRUCTUS CHAENOMELIS.

【基金】 Supported by Project of Hubei Provincial Education Department( B20101201);Project of Yichang Science & Technology Bureau( 2010A01301-04);Scientific Research Project of China Three Gorges University ( 0620080702)
  • 【分类号】R284.1
  • 【下载频次】24
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