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Extraction, Identification and Content Determination of Polysaccharides from the Leaves of Prunus pseudocerasus Lindl.

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【作者】 LIU De-shengYAN LingHAN Jing-tian

【Author】 LIU De-sheng, YAN Ling, HAN Jing-tian* College of Pharmacy, Binzhou Medical College, Yantai, Shandong 264003, China

【机构】 College of Pharmacy, Binzhou Medical College

【摘要】 [Objective] To study the extraction, qualitative identification and quantitative assay of polysaccharides from the leaves of Prunus pseudocerasus Lindl. (PPP), so as to provide reference for its comprehensive application. [Method] Polysaccharides were extracted with the method of water exaction and alcohol precipitation after the leaves were defatted with 95% EtOH and petroleum ether. Then, the protein in PPP was removed by Sevag method. A series of experiments were used to identify the PPP, including Molish test (α-naphthol test), anthrone-sulfuric acid method test, fehling test, fourier transform infrared spectrometry (FT-IR), and iodine test. The content of PPP was determined by anthrone-sulphuric acid colrimetry method. [Result] P. pseudocerasus polysaccharides were grey white in color. The content of polysaccharides in P. pseudocerasus leaves was 29.7 mg/g. [Conclusion] The method is simple, sensitive, reproducible, and reliable.

  • 【分类号】TQ461
  • 【被引频次】1
  • 【下载频次】34
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