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Study on the Extraction Process of Alkaloids from HERBA LOBELIAE CHINENSIS with Compound Enzymes under Ultrasonic Wave

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【作者】 HUANG Xiu-xiang

【Author】 HUANG Xiu-xiang* Department of Chemistry and Life Sciences, Hechi University, Yizhou, Guangxi 546300, China

【机构】 Department of Chemistry and Life Sciences, Hechi University

【摘要】 [Objective] To study the optimal technical conditions of extracting alkaloids from HERBA LOBELIAE CHINENSIS with complex enzymes under ultrasonic wave. [Method] With ethanol as the solvent, single factor experiment and orthogonal test method were employed to test the effects of five factors on extraction of alkaloids, including cellulase dose, pectinase dose, extraction time, pH value, and ethanol concentration. [Result] The optimal extraction procedure was as followed: cellulase 24 mg, pectinase 45 mg, extraction time 30 min, enzymatic pH 5.4, and ethanol concentration 70%. [Conclusion] High in extraction rate and good in stability, the method favored the determination of alkaloids from HERBA LOBELIAE CHINENSIS.

【基金】 Supported by Research Project of Education Department of Guangxi Zhuang Autonomous Region ( 201010LX457)
  • 【分类号】R284.1
  • 【被引频次】2
  • 【下载频次】30
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