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Study on Establishment of the Clone of Centipeda minima

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【作者】 Shiying SONGTing JINGChang XUNa XUChangyang JIANG

【Author】 Shiying SONG,Ting JING,Chang XU,Na XU,Changyang JIANG * College of Life Sciences,Liaoning Normal University,Dalian,Liaoning 116029,China

【机构】 College of Life Sciences,Liaoning Normal University

【摘要】 [Objective]To study the tissue culture of the tender stem of Centipeda minima and provide an effective means to protect the wild C.minima resources.[Method]The tender stems of C.minima as samples were sampled for the induction and differentiation of adventitious buds,as well as the rooting,successive transfer culture,transplanting and plantation of tube seedlings.[Result]MS+0.4 mg/L 6-BA+0.1 mg/L NAA was the best medium for the direct induction and differentiation of adventitious buds from tender stems; MS+0.1 mg/L NAA+0.5 mg/L GA 3+0.6 mg/L 6-BA was the ideal medium for the proliferation of adventitious buds; the best method for the rooting of adventitious buds was to immerse the bottom incision of adventitious buds into 40 mg/L IAA solution for 6 min,and then inoculate it into the N 6 +0.2 mg/L NAA medium for rooting.The survival rate of transplanted and planted tube seedlings maintained at high level,and the planted tube seedlings maintained all botanical traits of wild C.minima resources.[Conclusion]An asexual clone system of C.minima is established for its artificial cultivation,which provides an effective means to satisfy the market demands and the protection of wild C.minima resources.

【基金】 Supported by the Undergraduate Teaching Reform Program of Liaoning University Education (201203041-4);the Innovative and Entrepreneurial Training Project of College Students in Jiangsu Province (201203015011)
  • 【分类号】S567.219;R282.7
  • 【下载频次】13
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