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Extraction Technology of Polysaccharides from the Leaves of Coix lacryma-jobi L. by Cellulase Method

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【作者】 Tingqi PANQuantai WENHuafang XIAOCuixiao LANZudi YANSuoyi HUANG

【Author】 Tingqi PAN;Quantai WEN;Huafang XIAO;Cuixiao LAN;Zudi YAN;Suoyi HUANG;College of Clinical Laboratory Medicine,Youjiang Medical University for Nationalities,Baise;College of Pharmacy,Youjiang Medical University for Nationalities,Baise;

【机构】 College of Clinical Laboratory Medicine,Youjiang Medical University for NationalitiesCollege of Pharmacy,Youjiang Medical University for Nationalities

【摘要】 [Objective]To optimize the extraction technology of polysaccharides from the leaves of Coix lacryma-jobi L.by cellulase method.[Methods]Effects of pH value,cellulase dosage,extraction time and hydrolysis temperature on the extraction rate of polysaccharides were researched by single factor test and orthogonal test.[Results]The optimal extraction technology of polysaccharides in C.lacryma-jobi leaves was as follows:was 5 pH,50℃hydrolysis temperature,2.5 h extraction time,and 500U cellulase in 1 g powder of C.lacryma-jobi leaves.Under this extraction technology,the average extraction rate of polysaccharides was 14.45%and RSD was 0.18%.[Conclusions]Extracting the polysaccharides from C.lacryma-jobi leaves by cellulase method had the advantages of time saving and high efficiency.

【基金】 Supported by the National Natural Science Foundation of China(No.81360684);the Guangxi Natural Sciences Found Project(2011GXNSFA018046);the Special Found Project of Guangxi Traditional Chinese Medical Science and Technology(GZKZ 10-128);the Project of Guangxi Education Department(201106LX425);the Project of Guangxi Administration of Traditional Chinese Medicine(gzzc1047);the Special Project of Open Found of Guangxi Medical Experimental Research Center(KFJJ2011-04);College Student Innovation Training Project of Guangxi Zhuang Autonomous Region of China([2012]90);the Founding Project of Ethnic Medicine Association of Youjiang Medical University for Nationalities(20110715)
  • 【分类号】R284
  • 【下载频次】36
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