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Ferulic Acid Content of Angelica pubescens in Three Gorges Reservoir Area

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【作者】 Yingquan FANG

【Author】 Yingquan FANG;Chongqing Three Gorges Medical College;

【机构】 Chongqing Three Gorges Medical College

【摘要】 [Objective]To study the quality standard of angelicae pubescentis radix,which is a genuine medicinal material of Three Gorges Reservoir Region,focusing on the establishment of the content determination method for ferulic acid in angelicae pubescentis radix.[Method]Using the ferulic acid content as the index,the ferulic acid content was determined by HPLC method,the optimum chromatographic conditions for the chromatographic column was TSK-GEL DDS-C18(4.6 mm×150 mm,5μm),mobile phase of anhydrous methanol-water-phosphoric acid(30∶70∶0.3,V/V/V),the flow rate of 1.0 mL/min,the detection wavelength of 320 nm,and the column temperature of40℃.[Results]The ferulic acid showed a good linear relationship in the range of 0.006-0.15μg/mL,and the regression equation was y=4 564 754x-(3 882.699,r=0.999 9),and the RSD of precision test was 0.67%;the RSD of peak area of stability test was 1.11%;the contents of 6 parallel ferulic acid samples were obtained by repeatability test,and the RSD was 1.20%;the contents of ferulic acid of a batch of concentrated Angelica were determined for 6 times in reproducibility test and the RSD was 0.93%;the recovery rate of 6 sampling obtained by recovery test was between 94.63%-99.51%,and the RSD was 1.67%,is less than 3%,indicating that the method is reliable.[Conclusion]the determination method for the contents of ferulic Angelica pubescens medicinal acid.The method is simple,rapid and accurate,reproducible,and effective control of Angelica pubescens medicinal materials quality.

  • 【分类号】R284
  • 【下载频次】38
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