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HPLC Analysis for Fingerprinting of Bidens Genus Plants

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【作者】 Qing XIAYuan LIU

【Author】 Qing XIA;Yuan LIU;Sichuan College of Traditional Chinese Medicine;Ethnic Pharmaceutical Institute of Southwest University for Nationalities;

【机构】 Sichuan College of Traditional Chinese MedicineEthnic Pharmaceutical Institute of Southwest University for Nationalities

【摘要】 Objective]To establish an HPLC method for fingerprint of Bidens genus plants,for distinguishing five species of this genus.[Methods]Ten batches of Bidens genus medicinal materials were used for HPLC analysis.The chromatic parameters were as follows:Kromasil C18(4.6 mm×250 mm,5μm)as chromatic column,absolute methanol-0.1%phosphoric acid as mobile phase(gradient dilution),flowing speed at 1 mL/min,detection wavelength at 290 nm,column temperature at 30℃and injection amount of 10μL.[Results]The HPLC fingerprint for discriminating Bidens genus plants was established by using similarity software analysis.The medicinal materials tested were classified into five categories by primary ingredient analysis and phylogenetic analysis.[Conclusion]This method is simple,stable,repeatable and feasible,and it can well separate most of the chemical ingredients,which provides scientific and efficient method for discriminating Bidens genus medicinal materials.

【基金】 Supported by the National Major Scientific and Technological Special Projectof the State Ministry of Science and Technology(2011ZX09307-002-01);the China Ministry of Science and Technology(No.2012BAI27B07);National Natural Science Foundation of China(No.81173653);the Research Funds of Southwest University for Nationalities(13NLY01)
  • 【分类号】S567.219
  • 【下载频次】18
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