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Effect of Polygonum Perfoliatum On the Expression of HBsAg and HBeAg in HepG2.2.15 Cells

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【作者】 Ya GAOKefeng ZHANG

【Author】 Ya GAO;Kefeng ZHANG;Guiin Medical University;

【机构】 Guilin Medical University

【摘要】 [Objective]To study the effect of alcohol extraction of Polygonum Perfoliatum on the expression of HBsAg and HBeAg in HepG2.2.15cells.[Methods]The half toxic concentration(TC50)and the maximum non-toxic concentration(TC0)of Polygonum Perfoliatum extracts to HepG2.2.15 cells can be detected via methyl thiazolyl tetrazolium(MMT)method.With enzyme-linked immunosorbent assay(ELISA)method,the secretion level of HBsAg and HBeAg in cell supernates could be measured after a 72-hour action of drugs with four different concentrations on the HepG2.2.15 cells.[Results]Liquids with concentration of 0.4,0.2 and 0.1 mg/mL have an inhibitory effect on the secretion of HBsAg and HBeAg after being cultured for 72 h in the cell strain of HepG2.2.15 and there is a dose-response relationship between the concentration and the effect.Therapeutic indexes of the liquids were all greater than 1 but lesser than 2,which showed that alcohol extract of Polygonum Perfoliatum was an effective therapeutic drug of low toxicity.[Conclusions]Alcohol extraction of Polygonum Perfoliatum L.has an anti-HBV effect in vitro and certain toxicity as well.

  • 【分类号】R285
  • 【被引频次】1
  • 【下载频次】36
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