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Preliminary Research on the Inducing Effects of Wogonin on the Apoptosis of Lung Cancer Cells and Its Mechanism

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【作者】 Kaifei HUANGYiqi HUANG

【Author】 Kaifei HUANG;Yiqi HUANG;Centre for Drug Research and Development,Institute of Medicine in Xiamen;

【机构】 Centre for Drug Research and Development,Institute of Medicine in XiamenCentre for Drug Research and Development,nstitute of Medicine in Xiamen

【摘要】 [Objective]To investigate the effects of wogonin on the proliferation and apoptosis of lung cancer cell lines A549 and explore its molecular mechanism.[Methods]MTT method was used to evaluate the effects of wogonin on the proliferation of A549;Hochest staining method and flow cytometer were used to evaluate the effects of wogonin on the apoptosis of cells;Western-blotting detector was used to evaluate the effects of wogonin on the apoptosis of related proteins.[Results]Wogonin can inhibit the growth of A549 cells,and its inhibitory rate of cell proliferation was dependent on the drug concentration and the reaction time.According to the results of morphological observation,wogonin can obviously lead to the apoptosis of lung cancer cells A549,and inhibit the expression of apoptosis-related proteins Bcl-2 and survivin,as well as improve the expression of Bax protein.[Conclusions]Wogonin can apparently induce the apoptosis of lung cancer cells A549,and its molecular mechanism of inducing apoptosis was likely to be associated with the down-regulation of Bcl-2 and the expression of survivin protein.

【基金】 Supported by Natural Science Fund of Fujian Province(2012D047);Innovation Project of Fujian Province Health Department(2009-CXB-70);Youth Fund of Fujian Province Health Department(20102115)
  • 【分类号】R285
  • 【下载频次】19
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