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Study on Inhibiting Effect of Ursolic Acid on Growth and Apoptosis of Human Hepatocarcinoma Cell Line SMMC-7721

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【作者】 Lifang CHENGXia XUBingbing CUIHong CHENGLijing HOUQing YANGJin LIUYanling MUXuesheng YANKeming LI

【Author】 Lifang CHENG;Xia XU;Bingbing CUI;Hong CHENG;Lijing HOU;Qing YANG;Jin LIU;Yanling MU;Xuesheng YAN;Keming LI;Shandong Research Academy of Traditional Chinese Medicine;Shandong Chest Hospital;Traditional Chinese Medicine of Shandong University;Blood center of Shandong Province;Institute of Materia Medica Shandong Academy of Medical Sciences;

【机构】 Shandong Research Academy of Traditional Chinese MedicineShandong Chest HospitalTraditional Chinese Medicine of Shandong UniversityBlood center of Shandong ProvinceInstitute of Materia Medica Shandong Academy of Medical Sciences

【摘要】 [Objective]To explore the inhibiting effect of ursolic acid on human hepatocarcinoma cell line SMMC-7721. [Methods]Human hepatocarcinoma cell line SMMC-7721 was co-cultured with ursolic acid of different concentrations. After cultured for 24 and 72 h,the absorbancy was determined by neutral red stain. And with it,we could evaluate the inhibiting effect of ursolic acid on the proliferation of hepatoma cell line. The effect of ursolic acid on cell apoptosis and cell cycle of hepatoma carcinoma cell was determined by flow cytometry. [Results] The inhibiting effect of ursolic acid on the proliferation of SMMC-7721 cell was significant( P < 0. 05 or P < 0. 01) and it was time dependent and dose-dependent. The median inhibitory concentrations( IC 50) of 24 and 72 h were 12.59 and 8.32 mg/L. The detection result of flow cytometry indicated that apoptosis rate of ursolic acid on human hepatocarcinoma cell line SMMC-7721 reached 14. 07%. And the apoptosis rate rose with the increase of drug concentration. Ursolic acid could block the S phase of SMMC-7721 cell and its effect was positively correlated with dose. [Conclusions] Ursolic acid could significantly inhibit the proliferation of human hepatocarcinoma cell line SMMC-7721. And the antitumor effect of which might be correlated with its effect of inducing cell apoptosis and blocking cell cycle to S phase.

【基金】 National Special Project of New Medicine Development(2010ZX09401);Shangdong Science and Technology Key Project(2011GSF11902)
  • 【分类号】R735.7
  • 【下载频次】55
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