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Optimization by Refluxing Extraction of Total Flavonoids from the Leaves of Rubus coreanus Maq. var. corweanus

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【作者】 Huamei JIANGXiangqian WANGChunmei SUJingjing ZHANGJunqiang ZHANGGuishu ZHANG

【Author】 Huamei JIANG;Xiangqian WANG;Chunmei SU;Jingjing ZHANG;Junqiang ZHANG;Guishu ZHANG;College of Chemistry and Material Engineering;Library of Guiyang University;

【机构】 College of Chemistry and Material EngineeringLibrary of Guiyang University

【摘要】 [Objective]This paper explains screening the optimal condition for extracting total flavonoids from the leaves of Rubus coreanus. [Methods]With the yield of total flavonoids content set to be the indicator,4 factors,concentration of extraction agent,solid-liquid ratio,refluxing temperature and extraction duration,were selected by means of orthogonal array design in order to carry out L 9( 34) orthogonal array design. And the content of total flavonoids was determined by spectrophotometry. [Results] The conditions of optimum extraction process were 70%- concentration ethyl alcohol,1∶ 10( g / mL) solid-liquid ratio,80 ℃ of refluxing temperature,and 3 h of extraction duration. Under these conditions,the content of total flavonoids extracted from Rubus coreanus leaves could be 44. 931 3 mg / g,far more than 202. 79 mg /100 g that was reported in the previous documents. [Conclusions] It was verified by the experiment that total flavonoids extracted from of Rubus coreanus leaves by this process was of good stability and reproductibility and had a high rate of sample recycling and certain feasibility.

【基金】 Supported by Mutual Fund Project of Sichuan Department of Science and Technology and Guiyang College(LKG[2013]13);Key Project of Teaching Contents and Curriculum System Reform of Guizhou Province Education Department in 2013([2013]446);Guiyang College Teaching Quality and Teaching Reform Project of Guizhou Province(201212180002)
  • 【分类号】S567.19
  • 【下载频次】28
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