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Effect of Extracting Solution of Euphorbia fischeriana Steud. on the Proliferation and Apoptosis of K562 Cell Lines

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【作者】 Donghua ZHANGGuiqing YANGSixiang LIUDongning SUNShanyi WANG

【Author】 Donghua ZHANG;Guiqing YANG;Sixiang LIU;Dongning SUN;Shanyi WANG;College of Combine Traditional Chinese and Western Medicine,Binzhou Medical College;National Key Specialty of Combined Traditional Chinese Medicine and Western in Tumor,107 Hospital of National Liberation Army;

【机构】 College of Combine Traditional Chinese and Western Medicine,Binzhou Medical CollegeNational Key Specialty of Combined Traditional Chinese Medicine and Western in Tumor,107 Hospital of National Liberation Army

【摘要】 [Objective]To observe the effect that extracting solution of radix euphorbiae lantu has on the proliferation and apoptosis of K562 cell lines. [Methods] The growth inhibition ratio of the extracting solution of Euphorbia fischeriana Steud. on K562 cell line was determined by MTT. Apoptosis and its morphology were observed by Hoechst fluorescence staining. Changes of cell membrane potential and cell cycle were detected by FCM after treated with extracting solution of Euphorbia fischeriana Steud. [Results]The extracting solution of Euphorbia fischeriana Steud. had significant inhibiting effect on the growth of K562 cell line. Observed by fluorescence microscope,we found that the extracting solution of Euphorbia fischeriana Steud. could change the morphology of K562 cell. The mitochondrial transmembrane potential( △ψm) lowered and the number of cells in G0/ G1phase increased significantly which were detected by FCM. [Conclusions] The extracting solution of Euphorbia fischeriana Steud. could promote the apoptosis of K562 cell line and inhibit its growth by changing the cell cycle.

【基金】 Science and Technology Key Project of the Entire Army(06G034)
【所属期刊栏目】 Pharmacological and Toxicological Analyses (2014年02期)
  • 【分类号】S567.239
  • 【下载频次】12
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