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HPLC Fingerprint of Pyrrosia calvata (Bak.) Ching

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【作者】 Yan HUANGYoumin WENJunhua MABuming LIULu CHENMingsheng CHEN

【Author】 Yan HUANG;Youmin WEN;Junhua MA;Buming LIU;Lu CHEN;Mingsheng CHEN;Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards;Guangxi Institute of Traditional Medical and Pharmaceutical Science;Wantong Pharmaceutical Co. ,Ltd.;

【机构】 Guangxi Key Laboratory of Traditional Chinese Medicine Quality StandardsGuangxi Institute of Traditional Medical and Pharmaceutical ScienceWantong Pharmaceutical Co. ,Ltd.

【摘要】 [Objective]To establish the fingerprint of Pyrrosia calvata( Bak.) Ching for evaluating its quality by HPLC. [Methods] The HPLC analysis was carried out on SinoChrom ODS-BP( 5 μm,4. 6 mm × 250 mm) column with mobile phase of acetonitrile- 0. 2% phosphoric acid solution( gradient elution) at the flow of 1. 0 mL / min,and the detection wavelength was 205 nm. [Results]The established fingerprint of P. calvata was used to determine 10 batches of sample from different sources. 12 common peaks were confirmed as the character fingerprints through similarity analysis on the fingerprint of the P. calvata. [Conclusions]The method had good precision,stability and reproducibility, providing a scientific base for controlling the quality of P. calvata by establishing fingerprint of the P. calvata.

【基金】 Supported by the Key Scientific and Technological Project of Guangxi(1099062-4);the Technology Innovation Project of Nanning(2009-2-15)
【所属期刊栏目】 Chemical Composition of Natural Medicine (2014年02期)
  • 【分类号】S567.239
  • 【下载频次】18
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