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Contents of Forsythin and Forsythiaside A in Different Parts of Forsythia suspensa and Its Medicinal Value

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【作者】 Jinming WANGShengci FANAnping LITingting CHENBin SUNXia YANG

【Author】 Jinming WANG;Shengci FAN;Anping LI;Tingting CHEN;Bin SUN;Xia YANG;Shanxi Zhendong Pharmaceutical Co. ,Ltd.;

【机构】 Shanxi Zhendong Pharmaceutical Co. ,Ltd.

【摘要】 [Objective]To compare the contents of forsythin and forsythiaside A in different parts of Forsythia suspensa( Thunb.) Vahl.,and to provide scientific references for the development and utilization of F. suspensa resources. [Methods] Contents of forsythin in the fruits, leaves,branches and fruit stems of F. suspensa were detected by HPLC. Chromatographic column was Luna5uC18( 2) 100A( 250 mm ×4.6 mm, 5 μm). As for forsythin,the mobile phase was acetonitrile- water( 25∶ 75,V / V); detection wavelength was 277 nm; column temperature was room temperature and flow rate was 1. 0 mL / min. As for forsythiaside A,the mobile phase was acetonitrile- 0. 4% glacial acetic acid solution( 15∶85,V/V); detection wavelength was 330 nm; column temperature was room temperature and flow rate was 1.0 mL/min. [Results]Forsythin had good linear relationship within 3. 33- 13. 32 μg / mL( R2= 0. 999 7); the average recovery rate was 99. 30% and the RSD was 1. 4%. Forsythiaside A had good linear relationship within 4. 17- 6. 69 μg / mL( R2= 0. 999 7); the average recovery rate was 98. 26% and the RSD was 1. 8%. [Conclusions]Forsythin content was the maximum in leaves,followed with fruits and branches; and its content was the minimum in fruit stems. Forsythiaside A content was also the maximum in leaves,followed with fruits and fruit stems; and branches had the minimum content.

【基金】 Supported by the Scientific and Technological Support Project during the"12th Five-Year Plan"(2011BA107B01);Key Science and Technology Program of Shanxi Province(20120313015-9);the Science and Technology Planning Project of Changzhi City(20118033-1)
【所属期刊栏目】 Chemical Composition of Natural Medicine (2014年02期)
  • 【分类号】S567.79
  • 【被引频次】1
  • 【下载频次】30
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