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Simultaneous Detection of Four Index Components in Banxia Xiexin Decoction by RP-HPLC

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【作者】 Xiaoyan ZHAOJingxia WANGYunzhang XUYuan LIUJizhong ZHANG

【Author】 Xiaoyan ZHAO;Jingxia WANG;Yunzhang XU;Yuan LIU;Jizhong ZHANG;Ethnic Medicine Institute,Southwest University for Nationalities;

【机构】 Ethnic Medicine Institute,Southwest University for Nationalities

【摘要】 [Objective]To establish the simultaneous detection method for four index components in Banxia Xiexin Decoction by RP-HPLC,which were baicalin,glycyrrhizic acid,glycyrrhetinic acid and oleanolic acid.[Methods]Diamonsil C18(2)chromatographic column(4.6 mm×250 mm,5μm)was adopted.Mobile phase was methanol(A)-0.5%phosphoric acid aqueous solution(B)with gradient elution;flow rate was 1.0 mL/min;column temperature was 30℃;detection wavelength was 230 nm,and injection volume was 20μL.[Results]The detection limits of baicalin,glycyrrhizic acid,glycyrrhetinic acid and oleanolic acid were 0.031,0.025,0.004 and 0.007μg,respectively.And the baicalin,glycyrrhizic acid,glycyrrhetinic acid and oleanolic acid all reached good isolation.Their linear ranges were0.330-16.50μg(r=0.999 5),0.347-17.37μg(r=0.999 0),0.035-1.74μg(r=0.998 4)and 0.027-1.330μg(r=0.999 3);and the average recovery rates were 101.4%(RSD=1.36%),98.5%(RSD=1.07%),96.9%(RSD=0.84%)and 99.0%(RSD=1.79%).[Conclusions]This method was simple,stable,precise and practical,and could be used for the simultaneous detection of four index components in Banxia Xiexin Decoction.

【基金】 Supported by the National Science and Technology Support Project of China(2012BAI27B07);the 2012 Fundamental Research Funds for the Central Universities(12NZYTH02)
【所属期刊栏目】 Chemical Composition of Natural Medicine (2014年03期)
  • 【分类号】S853.7
  • 【下载频次】16
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