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Effects of Resveratrol on Proliferation and Cell Cycle of HepG-2 Cell

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【作者】 Shengjiu GUDan CHENJunbin QIMeibo LIYourui XUKaimei ZHU

【Author】 Shengjiu GU;Dan CHEN;Junbin QI;Meibo LI;Yourui XU;Kaimei ZHU;College of Pharmacy,Guilin Medical University;Workers’ Hospital of Guangxi;

【机构】 College of Pharmacy,Guilin Medical UniversityWorkers’ Hospital of Guangxi

【摘要】 [Objective] To discuss the effects of resveratrol on the proliferation and cell cycle of human hepatoblastoma-derived HepG-2 cell line. [Methods]The resveratrol was extracted by microwave-assisted method and HepG-2 cells were exposed to resveratrol of different concentrations which were 12. 5,25,50 and 100 μmol /L. The inhibiting effect of resveratrol on the proliferation of HepG-2 cells was measured by MTT. Morphologic changes of cells were detected by the phase contrast microscope. The cell cycle of HepG-2 cells treated with resveratrol were analyzed by flow cytometry. [Results]Resveratrol could significantly inhibit proliferation of HepG-2 cells and the effect was time and concentration dependent. According to results of FCM,resveratrol could block HepG-2 cells into S phase and the effect was dose dependent. [Conclusions]Resveratrol could inhibit the proliferation of HepG-2 cells and induce apoptosis. It could induce cell blockage in S phase.

【基金】 Guangxi Scientific and Technological Key Project(GuangxiScience and Technology 129825-21);Science and Technology Project of Guangxi Education Department(201202ZD065,2013lX080,2013YB154);Guilin Scientific and Technological Key Project(Tackle Key Problems in Science and Technology 20130103-8,20130103-9,20130113-1,20120105-5;20120105-16;20120105-8)
【所属期刊栏目】 Pharmacological and Toxicological Analyses (2014年05期)
  • 【分类号】R735.7
  • 【下载频次】25
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