节点文献

Enzyme Extraction and Pre-column Derivation HPLC Analysis of Rose Polysaccharides

免费订阅

【作者】 Yuehui ZHANGLiwen HANLan ZHENGJianguo SHIJunhui YANGYaohong MAYingying DUANWenhong FENGShengmin ZHOUDapeng CAOWei ZHAO

【Author】 Yuehui ZHANG;Liwen HAN;Lan ZHENG;Jianguo SHI;Junhui YANG;Yaohong MA;Yingying DUAN;Wenhong FENG;Shengmin ZHOU;Dapeng CAO;Wei ZHAO;Key Laboratory for Biosensor of Shandong Province,Biology Institute,Shandong Academy of Sciences;Shandong Fuyang Biotechnology Co.ltd.;Shandong Institute of Light Industry;

【机构】 Key Laboratory for Biosensor of Shandong Province,Biology Institute,Shandong Academy of SciencesShandong Fuyang Biotechnology Co.ltd.Shandong Institute of Light Industry

【摘要】 [Objective] To screen the extraction method of rose polysaccharides,and to provide theoretical foundation for the reuse of residues after extracting the essential oils from rose. [Methods]Rose polysaccharides were extracted by enzyme method. Pre-column derivation HPLC was used to analyze the contents. [Results]The polysaccharides extracted from dry rose and rose residues by normal method were 98. 65 and50. 87 mg /g,respectively. After pretreated by enzyme,the polysaccharides were 124. 87 and 75. 99 mg /g. The monosaccharides in rose polysaccharides were analyzed by pre-column derivation HPLC. And the rose polysaccharides were mainly composed by mannose,rhamnose,galacturonic acid,glucose,galactose and arabinose. And their molar ratio was 1∶ 2. 2∶ 6. 0∶ 2∶ 7. 9∶ 8. 0. The sugar composition of primary structure of rose polysaccharides extracted by enzyme method was the same as that extracted by normal method(not adding enzyme). [Conclusions]Useful rose polysaccharides could be extracted from the residues by enzyme method.

【所属期刊栏目】 Chemical Composition of Natural Medicine (2014年05期)
  • 【分类号】R284;R285
  • 【下载频次】41
节点文献中: 

本文链接的文献网络图示:

浏览历史:
下载历史: