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Screening of High-yielding Strain for the Extraction of Total Saponins from Agave sisalana perr. ex Engelm and Its Optimal Fermentation Condition

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【作者】 Feifei ZHOUZaibin HAODongmei LIAOFei ZHANGKexian YIJinlong ZHENGXiuli WANG

【Author】 Feifei ZHOU;Zaibin HAO;Dongmei LIAO;Fei ZHANG;Kexian YI;Jinlong ZHENG;Xiuli WANG;College of Chemistry and Bioengineering,Guilin University of Technology;Guangxi Academy of Industrialization Engineering of Zhuang Medicine;Environment and Plant Protection Institute,CATAS;

【机构】 College of Chemistry and Bioengineering,Guilin University of TechnologyGuangxi Academy of Industrialization Engineering of Zhuang MedicineEnvironment and Plant Protection Institute,CATAS

【摘要】 [Objective] To screen the high-yielding strain for the extraction of total saponins from Agave sisalana perr. ex Engelm and its optimal fermentation condition.[Methods]Hundreds of soil samples were collected from the nearby mountains of Guilin City. Strain JMZ-N06 was obtain based on screening. Total saponins were extracted from A. sisalana residues by using strain JMZ-N06. L9( 34) orthogonal test was carried out with the factors of solid-liquid ratio,fermentation time,fermentation temperature and pH value of fermentation liquid.[Results] The optimal extraction technology was as follows: 1∶ 20 solid-liquid ratio( 50 g residues in 1 L fermentation liquid),60 h fermentation time,32 ℃fermentation temperature and pH 6. 0 of fermentation liquid.[Conclusions]Stain JMZ-N06 and the extraction technology were both stable; and stain JMZ-N06 was high high-yielding strain for the extraction of total saponins from A. sisalana.

【基金】 Supported by the National Natural Science Foundation of China(31060193,31200269);the Special Found for Technology System Construction of National Bast Fiber Industry(CARS-19);the Research Project of Guangxi Academy of Industrialization Engineering of Zhuang Medicine(2014GXZYCYH03)
【所属期刊栏目】 Processing of Traditional Chinese Medicine and Quality Control (2014年10期)
  • 【分类号】Q93
  • 【下载频次】39
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