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Mechanism of Anti- influenza Virus of Cinnamaldehyde and Volatile Oils from RAMULUS CINNAMOMI

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【作者】 Rong LIUTing HENan ZENGTian CHENLing GOUJinwei LIU

【Author】 Rong LIU;Ting HE;Nan ZENG;Tian CHEN;Ling GOU;Jinwei LIU;Pharmacy College,Chengdu University of Traditional Chinese Medicine;Department of Pathogenic Biology,Chengdu Medical College;

【机构】 Pharmacy College,Chengdu University of Traditional Chinese MedicineDepartment of Pathogenic Biology,Chengdu Medical College

【摘要】 [Objective] To observe the direct killing effects of cinnamaldehyde and volatile oils from RAMULUS CINNAMOMI on inoculated mouse-lung adapted strains of influenza virus A / PR /8 /34( H1N1),and to discuss the TLR7 signaling mechanism of their anti-influenza effects. [Methods]1 With Madin-Darby canine kidney( MDCK) infected by influenza virus as the vector,the effective concentration( IC50)and therapeutic index( TI) of the two drugs were detected in inhibiting the proliferation influenza virus in vitro. 2 Effects of two drugs on the IFN-β secretion of infected MDCK cells were detected by ELISA method. 3Effects of two drugs on the TLR3,TLR7,TRAF-3,IRAK-4 and IFN-β mRNA expression of infected MDCK cells were observed by Real-Time PCR method. [Results]1 Cinnamaldehyde and volatile oils of RAMULUS CINNAMOMI had significant direct killing effects on influenza virus; their IC50 were 5. 77 × 10- 7and 1. 85 × 10- 7m L / m L,and the TI were 9. 51 and 27. 04,respectively. 2 Compared with virus control group,0. 25 × 10- 5m L / m L cinnamaldehyde and volatile oils of RAMULUS CINNAMOMI could significantly enhance the IFN-β content in supernatant of infected cells( P < 0. 05). 3 Compared with virus control group,0. 25 × 10- 5m L / m L cinnamaldehyde and volatile oils of RAMULUS CINNAMOMI could significantly increase the TLR7,IRAK-4 and IFN-β mRNA expression levels of infected cells( P < 0. 05).[Conclusions] Cinnamaldehyde and volatile oils of RAMULUS CINNAMOMI showed significant inhibitory effects on the proliferation of H1N1 influenza virus. Its antiviral mechanism might be related to the stimulation of TLR7 pathway,the activation of IRAK-4,and the high expression of IRAK-4.

【基金】 Supported by the National Natural Science Foundation of China(30973923,8173637);the 2010 Project for High-level Overseas Talents([2010]411,412);Cultivation Project of Sichuan Education Department(12ZB037)
【所属期刊栏目】 Pharmacological and Toxicological Analyses (2015年Z1期)
  • 【分类号】R285
  • 【下载频次】50
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