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Extraction and Content Detection of the Total Flavonoids in Different Parts of Leontopodium franchetii Beauv. at Different Harvest Periods

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【作者】 Xiaoyan ZHAOYun TENGYijun CHENPanpan LIUYuan LIU

【Author】 Xiaoyan ZHAO;Yun TENG;Yijun CHEN;Panpan LIU;Yuan LIU;School of Chemistry and Environmental Protection Engineering;Ethnic Medicine Institute,Southwest University for Nationalities;

【机构】 School of Chemistry and Environmental Protection EngineeringEthnic Medicine Institute,Southwest University for Nationalities

【摘要】 [Objective] To verify the correctness of " the harvest period of Leontopodium franchetii Beauv. was flowering period and the medicinal part was leaves and flowers ". [Methods] With rutin as the reference substances,total flavonoids contents in different parts of L. franchetii at different harvest periods were detected. The detection wavelength was 512 nm. Effects of different factors on the extraction rate of total flavonoids were investigated,such as extraction solvents,solid-liquid ratio,extraction methods and extraction times. And the optimal extraction technology for total flavonoids was selected.[Results]The optimal extraction technology for total flavonoids from L. franchetii was as follows: 70% ethanol,1 g: 50 m L solid-liquid ratio,three extraction times,water-bath reflux extraction after ultrasonic-assisted extraction at 35 ℃ for 0. 5 h. Under this condition,the extraction rate of total flavonoids in L. franchetii leaves at flowering period reached as high as 310. 441 mg / g. Content of total flavonoids at flowering period was significantly higher than that of fruiting period. There were great differences in the content of total flavonoids in different parts of L. franchetii. Contents of total flavonoids in L. franchetii leaves and flowers at flowering period were significantly higher than those in other parts.[Conclusions] The contents of total flavonoids in different parts of L. franchetii at different harvest periods were detected and compared,so as to select the optimal harvest period( flowering period),which was consistent with the " flowering phase was the optimal harvest period of Tibetan herb L. franchetii for fire moxibustion treatment"; and the optimal medicinal parts were leaves and flowers. Therefore,total flavonoids could be used as the evaluation index for the optimal harvest period and medicinal parts of L. franchetii,which provided test foundation for the utilization and quality control of total flavonoids in L. franchetii.

【基金】 Supported by the National Science-Technology Support Plan Projec(2012BA127B07);the Project of Postgraduate Degree Construction,Southwest University for Nationalities(No.2015XWD-S0703)
【所属期刊栏目】 Chemical Composition of Natural Medicine (2015年Z2期)
  • 【分类号】Q946;S567.239
  • 【下载频次】28
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