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The Condition Selection of TLC Identification of Astragaloside Ⅳ in Jin Weng Zhili Particle

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【作者】 Tingting YANGQiumin RENYinsong RANZhenhua SUNQiang YIWenjie ZHONGJuan LIU

【Author】 Tingting YANG;Qiumin REN;Yinsong RAN;Zhenhua SUN;Qiang YI;Wenjie ZHONG;Juan LIU;The Rongchang Campus of the Southwestern University Veterinary Science Research Center of Chinese Medicine Innovation Laboratory;Veterinary Research Institute of Sichuan Baer Agriculture and Animal Husbandry Group;

【机构】 The Rongchang Campus of the Southwestern University Veterinary Science Research Center of Chinese Medicine Innovation LaboratoryVeterinary Research Institute of Sichuan Baer Agriculture and Animal Husbandry Group

【摘要】 [Objective] To screen optimum conditions of assay method and thin-layer chromatography was used for qualitative discrimination of astragaloside Ⅳ contained in Jin Weng Zhili particle,which provide basis for quality standard. [Methods] According to method described in Chinese Veterinary Pharmacopoeia,thin-layer chromatography was used for qualitative discrimination of astragaloside Ⅳ contained in Jin Weng Zhili particle by improving extraction method and adjusting the proportion of ingredients in developing solvent. [Results] For identification of astragaloside Ⅳ in Jin Weng Zhili particle using thin-layer chromatography,the results were the optimum after chromatographic condition was improved,ie,extraction,column transferring,developing solvent of trichloromethane-methyl alcohol-water( 10 ∶ 5 ∶ 1) which is better than method described in Chinese Veterinary Pharmacopoeia. [Conclusions] The method is of good feasibility,repeatability and specificity which is benefit to the identification of astragaloside Ⅳ contained in Jin Weng Zhili particle using thin-layer chromatography.

【基金】 Special Project of National Public Benefit Industry(agriculture)(201303040-05);Special Project of Science and Technology Groundwork(2013FY110600-03)
【所属期刊栏目】 Chemical Composition of Natural Medicine (2015年Z2期)
  • 【分类号】R284
  • 【下载频次】25
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