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Detection of Chlorogenic Acid,Macranthoidin B and Dipsacoside B in FLOS LONICERAE Grown in Guizhou Province by UPLC-PDA-ELSD

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【作者】 Liucun ZHAOGuanqing WENLi LIShuaihui ZHANGShiyun YE

【Author】 Liucun ZHAO;Guanqing WEN;Li LI;Shuaihui ZHANG;Shiyun YE;Yizhou Hospital of Traditional Chinese Medicine;Guiyang University of TCM;

【机构】 Yizhou Hospital of Traditional Chinese MedicineGuiyang University of TCM

【摘要】 [Objective] To detect chlorogenic acid,macranthoidin B and dipsacoside B in FLOS LONICERAE grown in Guizhou Province by UPLC-PDA-ELSD,and to investigate the quality of FLOS LONICERAE grown in Guizhou Province. [Methods] ACQUITYUPLC BEH C18 chromatographic column( 2. 1 mm × 100 mm,1. 7 μm) was adopted; mobile phase was acetonitrile( A)- 0. 4% acetic acid water( B); gradient elution was as follows: 0- 2 min,7% A; 2- 2. 3 min,7%- 28% A; 2. 3- 10 min,28% A; 10- 10. 5 min,28%- 7% A; gradient flow rate was as follows: 0- 2 min,0. 35 m L / min; 2- 2. 3 min,0. 35- 0. 25 m L / min; 2. 3- 2. 6 min,0. 25- 0. 15 m L / min; 2. 6- 2. 8min,0. 15- 0. 1 m L / min; 2. 8- 4 min,0. 1- 0. 2 m L / min; 4- 4. 3 min,0. 2- 0. 3 m L / min; 4. 3- 4. 5 min,0. 3- 0. 35 m L / min; 4. 5-10. 5 min,0. 35 m L / min; PDA( ultraviolet detector) was used to detect the chlorogenic acid( 330 nm wavelength); ELSD( evaporative light- scattering detector) was used to detected the macranthoidin B and dipsacoside B; temperature of the drift tube was 60 ℃; sprayer was48 ℃; gas pressure was 137. 9 k Pa; and column temperature was 50 ℃. [Results] Chlorogenic acid,macranthoidin B and dipsacoside B showed good linear relationship within the ranges of 0. 304 5- 1. 522 5 μg / m L( R2= 0. 999 4),0. 449- 1. 436 8 μg / m L( R2= 0. 999 3)and 0. 044 6- 0. 223 μg / m L( R2= 0. 999 4),respectively. [Conclusions] This method could accurately detect the contents of chlorogenic acid,macranthoidin B and dipsacoside B in FLOS LONICERAE grown in Guizhou Province,effectively shorten the measurement time and save reagents,so that it was suitable to detect the quality of FLOS LONICERAE grown in Guizhou Province.

【基金】 Supported by Major Project for TCM Modernization of Guizhou Province[(2012)6010]
【所属期刊栏目】 Chemical Composition of Natural Medicine (2015年Z4期)
  • 【分类号】R284.1
  • 【下载频次】21
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