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Properties of Phenylalnine Ammonialyase in Dioscorea opposita‘Tiegun’ and Its Browning Control

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【作者】 Xiting ZHAOLiwei JIANGYannan LILin MAHuizhen WANGMingjun LI

【Author】 Xiting ZHAO;Liwei JIANG;Yannan LI;Lin MA;Huizhen WANG;Mingjun LI;College of Life Sciences,Henan Normal University;Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs,University of Henan Province;Green Crude Drugs Biotechnology Engineering Laboratory of Henan Province;

【机构】 College of Life Sciences,Henan Normal UniversityEngineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs,University of Henan ProvinceGreen Crude Drugs Biotechnology Engineering Laboratory of Henan Province

【摘要】 With Dioscorea opposita‘Tiegun’as the research materials,phenylalnine ammonialyase( PAL) characteristics related to browning and the effects of different inhibitors on PAL activity were studied by spectrophotometry. Research results showed that within the test range,the optimal p H was 8. 6 for PAL of D. opposita‘Tiegun’. PAL had poor tolerance to acid and alkali. The optimal temperature was 50 ℃,showing certain tolerance to high temperature. Even under incubation for 30 min at 80 ℃,there were still about 12% PAL activity. L-cysteine hydrochloride and citric acid had significant inhibitory effects on PAL; while phytic acid had no significant inhibitory effects on PAL activity; but phytic acid showed no significant inhibitory effects. Sodium chloride promoted the PAL activity. In order to effectively restrict the browning of fresh slices of D. opposita‘Tiegun’caused by PAL,processing and storage of D. opposita‘Tiegun’slices should be carried out under acidic condition( p H < 2. 0),low temperature( < 20 ℃) or high temperature( > 80 ℃). L-cysteine hydrochloride should be the first choice of antibrowning reagent without sulfite for fresh slices,followed with citric acid.

【基金】 Supported by the National Major Scientific and Technological Special Project for‘Significant New Drugs Development’(2009ZX09308-002);the Key Scientific and Technological Project of Henan Province(112102210300);the Natural Science Foundation of Education Department of Henan Province(2010A180015,2010B360002)
【所属期刊栏目】 Biotechnology (2015年Z4期)
  • 【分类号】S567.239
  • 【下载频次】26
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