节点文献

Construction of a Multi-functional T-DNA Insertion Vector

免费订阅

【作者】 Linsu ZHANGMeizhen WANGShanfa LU

【Author】 Linsu ZHANG;Meizhen WANG;Shanfa LU;Institute of Medicinal Plant Development,China Academy of Chinese Medical Sciences;Department of Pharmacy,Qiannan Medical College for Nationalities;

【机构】 Institute of Medicinal Plant Development,China Academy of Chinese Medical SciencesDepartment of Pharmacy,Qiannan Medical College for Nationalities

【摘要】 [Objective] Plant mutants have been widely used in gene function analysis. T-DNA insertion is a promising approach for mutant library construction. This study aimed to construct a multifunctional T-DNA insertion vector for generating mutant libraries of Salvia miltiorrhiza Bunge.[Methods] By using pBI121 as a starting vector,an intron sequence from S. miltiorrhiza was introduced. Four enhancers were directionally connected by digestion with Ban I restriction endonuclease. GUS gene promoter was removed and GUS gene was moved forward to construct the recombinant vector pBI-INTRON-GUS-4E.[Results] The constructed recombinant vector pBI-INTRON-GUS-4E harbors an intron sequence from S.miltiorrhiza,four enhancers,GUS gene and NPTII gene,which exhibits multiple functions such as activation tagging,gene(promoter) trapping and insertional mutagenesis.[Conclusions]The successfully constructed multifunctional T-DNA insertion vector provided the basis for generating mutant libraries of S.miltiorrhiza and other plant species.

【基金】 Supported by Research Fund for the Doctoral Program of Higher Education of China(20111106110033)
【所属期刊栏目】 Biotechnology (2016年Z2期)
  • 【分类号】S567.53;Q943.2
  • 【下载频次】20
节点文献中: 

本文链接的文献网络图示:

浏览历史:
下载历史: