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Optimization of Ultrasound-assisted Extraction of Polyphenols from Periploca forrestii Schltr and Its Anti-allergic Activities

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【作者】 Enming HUHuaguo CHENXin ZHOUQingfang DENGXingdong WUSushan LUChao ZHAOXiaojian GONG

【Author】 Enming HU;Huaguo CHEN;Xin ZHOU;Qingfang DENG;Xingdong WU;Sushan LU;Chao ZHAO;Xiaojian GONG;Key Laboratory for Information System of Mountainous Area and Protection of Ecological Environment,Guizhou Normal University;Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of Medicine;The Research Center for Quality Control of Natural Medicine,Guizhou Normal University;

【机构】 Key Laboratory for Information System of Mountainous Area and Protection of Ecological Environment,Guizhou Normal UniversityGuizhou Engineering Laboratory for Quality Control & Evaluation Technology of MedicineThe Research Center for Quality Control of Natural Medicine,Guizhou Normal University

【摘要】 [Objective] To optimize the extraction of total polyphenols from Periploca forrestii Schltr. [Methods]Response surface methodology was applied. A three-level,three-variable Box-Behnken Design was employed to obtain the best possible combination of extraction solvent( ethanol concentration 40%-80%),extraction time( 10 min- 50 min) and ratio of solution to raw material(10∶ 1- 15∶ 1,v/w) for maximum extraction yield oftotal polyphenols. Besides,anti-allergic capacity of P. forrestii polyphenols was evaluated by initiation ratio of hyaluronidase decomposition of sodium hyaluronate. [Results]The results showed that optimized extraction conditions wereethanol concentration57.33%,extraction time 33. 33 min and ratio of solvent to raw material 16. 11∶ 1. Under these conditions,the experimental yield was 6. 03 ± 0.10%,which is well in close agreement with the value( 6. 1%) predicted by RSM model. [Conclusion]Pharmacological test showed that P.forrestii polyphenols have excellent anti-allergic activity.

【基金】 Supported by National Natural Science Foundation of China(81360641);Guizhou Provincial Research and Development of Modernization and Technology Industry of TCMs([2013]3014);Guizhou Province Development and Reform Commission of High-tech Projects([(2013)2069]);Characteristic Key Laboratory of Guizhou Province Education Department(KY[2012]005);Guizhou Province High Level Creative Talents Cultivation([2015]4033)
【所属期刊栏目】 Processing of Traditional Chinese Medicine and Quality Control (2016年Z2期)
  • 【分类号】R284.2
  • 【被引频次】1
  • 【下载频次】41
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