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Thin Layer Chromatography for Specnuezhenide in LIGUSTRI LUCIDI FRUCTUS- ASTRAGALI RADIX Extraction Solution

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【作者】 Maixun ZHUChuncheng YUGuowen CAOWengui FUShaoqin ZHAI

【Author】 Maixun ZHU;Chuncheng YU;Guowen CAO;Wengui FU;Shaoqin ZHAI;Chongqing Academy of Animal Sciences;Southwest University;

【机构】 Chongqing Academy of Animal SciencesSouthwest University

【摘要】 [Objective] To explore thin layer chromatography for specnuezhenide in LIGUSTRI LUCIDI FRUCTUS-ASTRAGALI RADIX extraction solution. [Methods] Ultrasonic was used to extract the active component specnuezhenide. Different extraction reagents were designed to obtain test sample solution. Specnuezhenide was taken as the reference substance. Ethyl acetate- acetone- formic acid- water were used to prepare different developing agents. 1% vanillin H2SO4 ethanol solution and 10% H2SO4 ethanol solution were used as color developing agent to conduct thin layer chromatography. [Results] The fourth extraction method indicated the best effect for the test sample solution of LIGUSTRI LUCIDI FRUCTUS- ASTRAGALI RADIX extraction solution. The developing agent was ethyl acetate- acetone- formic acid- water( 6∶ 3∶ 0. 5∶ 1). Color developing agent was 1% vanillin H2SO4 ethanol solution. In visible light,the test sample solution showed purple spot with the same color in corresponding position of the reference solution,and the developed color was clear. [Conclusion]The fourth method of thin layer chromatography for specnuezhenide in LIGUSTRI LUCIDI FRUCTUS- ASTRAGALI RADIX is used to prepare the test sample solution. The ethyl acetate-acetone-formic acid-water( 6∶ 3∶ 0. 5∶ 1) was used as the developing agent. After color developing using 1% vanillin H2SO4 ethanol solution,the same purple spot appears in the visible light and the corresponding position of the reference solution.

【基金】 Supported by Chongqing Agricultural Science and Technology Achievements Transformation Fund Project(cst2014jcsf-nycgzha10002);Chongqing Fundamental Research Project(15438)
【所属期刊栏目】 Biotechnology (2016年Z4期)
  • 【分类号】R284;O657.7
  • 【下载频次】28
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