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Determination of Tracheloside and Trachelogenin in Trachelospermum jasminoides (Lindl.) Lem. from Different Production Areas by RPHPLC

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【作者】 Kaishun WANGXuan LIUMuhua YUANYe ZHAOZhifeng ZHANG

【Author】 Kaishun WANG;Xuan LIU;Muhua YUAN;Ye ZHAO;Zhifeng ZHANG;School of Pharmacy,Southwest University for Nationalities;Institute of Qinghai-Tibetan Plateau,Southwest University for Nationalities;

【机构】 School of Pharmacy,Southwest University for NationalitiesInstitute of Qinghai-Tibetan Plateau,Southwest University for Nationalities

【摘要】 [Objective]To determine tracheloside and trachelogenin in Trachelospermum jasminoides( Lindl.) Lem. from different production areas by reverse phase high-performance liquid chromatography( RP-HPLC) method. [Methods] The experiment adopted ODS column( 4. 6mm × 250 mm,5 μm); the mobile phase was methanol-water solution; the volumetric flow rate was 1. 0 m L / min; the detection wavelength was 280 nm; the column temperature was 30℃. [Results]The highest content of tracheloside came from Chun’an of Zhejiang Province,followed by Tonglu of Zhejiang Province; the highest content of trachelogenin also came from Chun’an of Zhejiang Province,following by Lushan of Jiangxi Province. [Conclusions]The experiment results provide references for identification and application of Trachelospermum jasminoides( Lindl.) Lem.,and the established method is reliable,accurate and highly reproducible,and thus can be effectively applied in evaluating the quality of Trachelospermum jasminoides( Lindl.) Lem. from different production areas.

【基金】 Supported by Application Foundation Research Project of Sichuan Provincial Department of Science and Technology(2014JY0233);Science and Technology Innovation Program for Postgraduate of Southwest University for Nationalities(CX2016SZ050)
【所属期刊栏目】 Chemical Composition of Natural Medicine (2016年Z6期)
  • 【分类号】R284
  • 【下载频次】9
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