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Function of CoQ10 in Regulation of eN OS in High ox-LDL Induced HUVECs

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【作者】 Chan XUEWenqian LIMin GENGMingquan GAOXiaoming SIJingyu JINZhiwu HAN

【Author】 Chan XUE;Wenqian LI;Min GENG;Mingquan GAO;Xiaoming SI;Jingyu JIN;Zhiwu HAN;School of Pharmacy,Qingdao University;Department of Pharmacy,Affiliated Hospital of Qingdao University;

【机构】 School of Pharmacy,Qingdao UniversityDepartment of Pharmacy,Affiliated Hospital of Qingdao University

【摘要】 [Objectives] To study the function of coenzyme Q10( Co Q 10) in regulation of endothelial nitric oxide synthase( e NOS) in high oxidized low-density lipoprotein( ox-LDL) induced human umbilical vein endothelial cells( HUVECs). [Methods] HUVECs in exponential growth phase were inoculated to 96 orifice plate at the cell density of 1 × 10~4 cells / 100 μL. When cells were fused to 80%,Tween-80 group and Co Q 10 group were randomly divided. After acting 24 hours,cell survival rate was tested by CCK-8 method,to screen cytotoxicity of Tween-80 and Co Q 10. HUVECs were cultured and divided into the control group,model group,Co Q10 low concentration group,Co Q medium concentration group,and Co Q high concentration group. After drug action for 24 hours,except the control group,a1 l groups were given culture solution containing 35 μg / ml ox-LDL to reproduce proliferation model of HUVECs. Changes in expression of e NOS mRNA level were detected by RT-PCR( reverse transcription polymerase chain reaction),and changes in expression of e NOS protein were detected by Western blot.[Results]Tween-80 dilution 10000 times had no cytotoxicity,and the difference was not statistically significant( P > 0. 05); when Co Q 10 concentration was 60 μM,it had cytotoxicity,and the difference was statistically significant( P < 0. 05). Compared with the control group,the e NOS in model group declined in expression of both the mRNA and protein,and the difference was significantly significant( P < 0. 05); compared with the model group,the e NOS mRNA and protein rose in Co Q 10 low concentration,medium concentration and high concentration groups,and the difference was statistically significant( P < 0. 05). [Conclusions]Co Q 10 can mitigate oxidative injury through improving the expression of e NOS in HUVECs.

【基金】 Supported by Program of Regulation of Oleanolic Acid to UCP2/ROS/cyto C/AIF,PON2/PPAR/AP-1,PON2,UCP2/FGF-2/P53/TSP-1 Pathways(81274126);Program of Study on Gene Expression and Cell Culture during Digestive Tract Regeneration of Apostichopus japonicus(Selenka)(31272397);Program of Study on Correlation between ANP and Cardiovascular Complication of Diabetes(ZR2011CM041)
【所属期刊栏目】 Pharmacological and Toxicological Analyses (2016年Z6期)
  • 【分类号】R329.2
  • 【被引频次】1
  • 【下载频次】18
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