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Extraction of Herba Lycopi Polysaccharide and Study of Its Antioxidant Activity by Cellulase Method

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【作者】 Tao HUANGZhaoping ZHANGZudi YANYunda LIYuanhui LIYing HUANGTingzhang HUANGQiaorong YUMengyao LINWende LUSuoyi HUANG

【Author】 Tao HUANG;Zhaoping ZHANG;Zudi YAN;Yunda LI;Yuanhui LI;Ying HUANG;Tingzhang HUANG;Qiaorong YU;Mengyao LIN;Wende LU;Suoyi HUANG;Clinical College,Youjiang Medical University for Nationalities;College of Pharmacy,Youjiang Medical University for Nationalities;College of Pharmacy,Guangxi University of Chinese Medicine;Key Laboratory of Guangxi’s Colleges for the Study of Characteristic Ethnic Medicine in Youjiang River Basin;

【机构】 Clinical College,Youjiang Medical University for NationalitiesCollege of Pharmacy,Youjiang Medical University for NationalitiesCollege of Pharmacy,Guangxi University of Chinese MedicineKey Laboratory of Guangxi’s Colleges for the Study of Characteristic Ethnic Medicine in Youjiang River Basin

【摘要】 [Objectives] To optimize the extraction process of Herba Lycopi polysaccharide by cellulase,and study its antioxidant activity.[Methods]The single factor test and orthogonal test were conducted to study the effect of p H,enzyme dosage,enzymolysis temperature and extraction time on the extraction of Herba Lycopi polysaccharide. And the test was done about its scavenging effect on DPPH radicals and superoxide anions,and the antioxidant activity was evaluated. [Results] The optimum extraction process by cellulase method was as follows: p H value of 7; enzyme dosage of 4 ml; enzymolysis temperature of 40 ℃; extraction time of 2. 5 h. The average extraction rate of Herba Lycopi polysaccharide was 10. 70%,and RSD was 0. 17%. The antioxidant activity test showed that Herba Lycopi polysaccharide had a significant scavenging effect on radicals. [Conclusions] Herba Lycopi polysaccharides had strong antioxidant activity,and could provide a scientific basis for the development and utilization of Herba Lycopi medicinal resources.

【基金】 Supported by Key Traditional Chinese Medicine Discipline Construction Project of State Administration of Traditional Chinese Medicine during the 12th"FiveYear"Plan Period[Guo Zhong Yi Yao Ren Jiao Fa(2012)32];Guangxi Key Discipline Construction Project[Gui Jiao Ke Yan(2013)16];Guangxi College Science and Technology Innovation Ability Construction Project and Key Laboratory Project of Guangxi’s Colleges for the Study of Characteristic Ethnic Medicine in Youjiang River Basin[Gui Jiao Ke Yan(2014)14];Innovation and Entrepreneurship-Chinese Medicine Teaching Team Project at the Autonomous Regional Level,and Teaching Quality and Teaching Reform Project of Guangxi’s Colleges and Universities in 2016[Gui Jiao Gao Jiao(2015)93&(2016)6];College Students’ Innovative Training Project of Youjiang Medical University for Nationalities in 2014(XCXB201424)
【所属期刊栏目】 Processing of Traditional Chinese Medicine and Quality Control (2017年03期)
  • 【分类号】R284.2;R285
  • 【被引频次】2
  • 【下载频次】42
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