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Optimization of Extraction Process and Content Determination of Total Flavonoids from Sanguisorbae Radix and Carbonized Sanguisorba Root by Orthogonal Experimental Design

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【作者】 Fenglin WANGYunzhang XUJingxia WANGYuan LIU

【Author】 Fenglin WANG;Yunzhang XU;Jingxia WANG;Yuan LIU;School of Pharmacy,Southwest Minzu University;Tongren City Bijiang District Secondary Vocational School;Institute of Botany,The Chinese Academy of Sciences;Department of Wine and Food Engineering,Sichuan Technology Business College;Ethnic Medicine Institute,Southwest Minzu University;

【机构】 School of Pharmacy,Southwest Minzu UniversityTongren City Bijiang District Secondary Vocational SchoolInstitute of Botany,The Chinese Academy of SciencesDepartment of Wine and Food Engineering,Sichuan Technology Business CollegeEthnic Medicine Institute,Southwest Minzu University

【摘要】 [Objectives] To optimize the extraction process of total flavonoids from Sanguisorbae Radix and carbonized Sanguisorba root,compare quality of different batches of Sanguisorbae Radix,study the effects of processing on the content of flavonoids,and provide scientific basis for reasonable utilization of Sanguisorbae Radix. [Methods] Test samples were prepared by heating,refluxing,and extraction,the extraction process was optimized by orthogonal experiment design,color was developed by NaNO2-Al( NO3)3-NaOH,and total flavonoids were measured by UV method at the wavelength of 510 nm. [Results] The linear relationship of rutin was excellent in the concentration range of 0. 1248 mg/mL-0. 5712 mg/mL,R2= 0. 9997; the average recovery was 99. 67% and the RSD was 0. 70%. The optimum extraction conditions were as follows: the volume fraction of ethanol was 50%,the extraction temperature was 90℃,the extraction time was 90 min,and the solid-to-liquid ratio was 1∶ 20( g/mL). [Conclusions] After optimization of the extraction process,the extraction rate of total flavonoids in samples of Sanguisorbae Radix was significantly increased; there was certain difference in the content of total flavonoids between different batches of Sanguisorbae Radix and processed products; the total flavonoids significantly declines in carbonized sanguisorba root,and the influence of processing on its curative effect was to be further studied.

【基金】 Supported by National Science and Technology Project of the Ministry of Science and Technology in the 13th Five-Year Plan Period(2015BAC05B02);Key Technology R&D Program of Sichuan Province,China(2015SZ0034);Innovating Research Program of Postgraduates of Southwest Minzu University in2016(CX2016SZ038)
【所属期刊栏目】 Chemical Composition of Natural Medicine (2017年06期)
  • 【DOI】10.19600/j.cnki.issn2152-3924.2017.06.002
  • 【分类号】R284.2
  • 【被引频次】1
  • 【下载频次】58
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