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The Protective Effect of CoQ10 on ox-LDL——induced HUVEC Injury by AP-1 and PON2

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【作者】 Wenqian LIZhiwu HANChunbo WANGYantao HAN

【Author】 Wenqian LI;Zhiwu HAN;Chunbo WANG;Yantao HAN;School of Pharmacy,Qingdao University;Department of Pharmacy,Affiliated Hospital of Qingdao University;School of Basic Medicine,Qingdao University;

【机构】 School of Pharmacy,Qingdao UniversityDepartment of Pharmacy,Affiliated Hospital of Qingdao UniversitySchool of Basic Medicine,Qingdao University

【摘要】 [Objectives] To explore the protective effect and possible mechanisms of the coenzyme Q10( CoQ 10) on the human umbilical vein endothelial cell( HUVEC) injury induced by the oxidized low-density lipoprotein( ox-LDL). [Methods]With the human umbilical vein endothelial cells( HUVECs) cultured in vitro as the test target,the HUVECs were randomly divided into 5 groups: normal control group; model group; low concentration CoQ10 group( 12. 5); medium concentration CoQ10 group( 25); high concentration CoQ10 group( 50). The CCK-8 method was used to test the cell viability,and the drug concentration was screened in 60 μM of CoQ 10 toxic concentration; the total protein was extracted and Western blot was used to detect the protein expression of c-fos,c-jun and PON2; the RT-PCR method was used for determination of the content of c-fos,c-jun and PON2 mRNA. [Results]Compared with the normal group,the cell viability was significantly reduced in the 35 μg/m L high ox-LDL model group,and the cell injury was induced; compared with model group,after 12 h pre-protection,12. 5 μM,25 μM,50 μM CoQ10 could all significantly improve the survival of injured cells( P < 0. 05); compared with the normal group;the protein and mRNA levels of c-fos,c-jun,PON2 in the model group all declined; compared with the model group,the protein level and mRNA expression of c-fos,c-jun,PON2 in different CoQ10 groups increased to varying degrees( P < 0. 05). [Conclusions] CoQ 10 could reduce the HUVEC injury induced by high ox-LDL,and possible mechanism was achieved by upregulating the expression of AP-1 and PON2.

【基金】 Supported by Project of National Natural Science Foundation(81274126)
【所属期刊栏目】 Biotechnology (2017年06期)
  • 【DOI】10.19600/j.cnki.issn2152-3924.2017.06.005
  • 【分类号】R543.5
  • 【下载频次】12
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