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Extraction and Separation of Oxysophocarpine from Sophora alopecuroides by Reverse Three-phase Membrane Cycle Method

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【作者】 Kariyemu AIHAITIMirzat MAIMAITIArkin IBURAIM

【Author】 Kariyemu AIHAITI;Mirzat MAIMAITI;Arkin IBURAIM;College of Pharmacy,Xinjiang Medical University;School of Public Health,Xinjiang Medical University;Central Laboratory of Xinjiang Medical University;

【机构】 College of Pharmacy,Xinjiang Medical UniversitySchool of Public Health,Xinjiang Medical UniversityCentral Laboratory of Xinjiang Medical University

【摘要】 [Objectives] To establish the high performance liquid chromatography( HPLC) determination method for oxysophocarpine and optimize the extraction and purification technology of oxysophocarpine from Sophora alopecuroides by inverse three-phase membrane cycle.[Methods]Based on the single-factor experiment,the effects of aqueous phase and organic phase volume ratio,the concentration of sodium hydroxide,concentration of hydrochloric acid and extraction cycle time on extraction process of oxysophocarpine were investigated using orthogonal design method,to determine the optimal extraction process. [Results]The oxysophocarpine was determined by Shim-pack VP-ODS chromatographic column( 4. 6 mm × 250 mm,5 μm),mobile phase was methanol-0. 2% phosphoric acid aqueous solution( 7∶ 93),gradient elution,flow rate was 1 m L/min,the sample size was 5 μm,column temperature was 30℃,detection wavelength was 221 nm. Aqueous phase and organic phase volume ratio was 1∶ 1,hydrochloric acid concentration was 0. 3 mol/L,sodium hydroxide concentration was 0. 75 mol/L,water pump flow rate was 6 m L/min,60 min cycle time. The extraction rate of oxysophocarpine 98. 21 % within 60 min under the best experimental conditions. Oxysophocarpine has good linearity relationship within the range of 0. 01-0. 7 mg/m L( r~2= 0. 9978,n = 6),the respective average recovery rate was 97. 47%( RSD = 1. 95%). [Conclusions] This extraction process is simple in operation,the organic solvent has low consumption,and can be used for extraction of alkaloid.

【基金】 Supported by Project of National Natural Science Foundation(21162030)
【所属期刊栏目】 Processing of Traditional Chinese Medicine and Quality Control (2017年06期)
  • 【DOI】10.19600/j.cnki.issn2152-3924.2017.06.008
  • 【分类号】R284.2
  • 【下载频次】18
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