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Identification of Lablab Semen Album by DNA Barcode Technology

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【作者】 Huiming LUOJian RAOBingyi XIAOPing NIEHai LINYe DING

【Author】 Huiming LUO;Jian RAO;Bingyi XIAO;Ping NIE;Hai LIN;Ye DING;Hunan Institute for Drug Control;Pharmaceutical Quality Evaluation Engineering Research Center of Hunan Province;

【机构】 Hunan Institute for Drug ControlPharmaceutical Quality Evaluation Engineering Research Center of Hunan Province

【摘要】 [Objectives] To identify ITS2 barcode of Lablab Semen Album and its adulterants,and provide a new method for the identification of Lablab Semen Album. [Methods] The ITS2 sequence was amplified by PCR and sequenced bi-directionally. After splicing by Codon Code Aligner,the data were processed with the aid MEGA software to construct the cluster dendrogram( neighbor-joining,NJ tree). [Results]The ITS2 sequence of Lablab Semen Album had length of 218 bp; the constructed cluster dendrogram indicated that all species were monophyletic and could be distinguished from other species. [Conclusions] The ITS2 barcode can be used for rapid identification of Lablab Semen Album and its adulterants and this experiment further verified that DNA barcode technology is effective in identification of traditional Chinese medicines.

【基金】 Supported by Key Program for Sci-tech Plan of Hunan Province Science and Technology Department(2014SK2001);Sci-tech Project for Food and Drug Safety of Hunan Food and Drug Administration(Xiang Shi Yao Ke R201612)
【所属期刊栏目】 Processing of Traditional Chinese Medicine and Quality Control (2017年06期)
  • 【DOI】10.19600/j.cnki.issn2152-3924.2017.06.012
  • 【分类号】R282.71
  • 【下载频次】26
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