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Preparation of Acute Myocardial Infarction Model for SD Rats and Ventricular Remodeling

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【作者】 Kaihang SHIZhuoya JIAYanfang FANYanan ZhaoXiaohui ZHOUKai CAO

【Author】 Kaihang SHI;Zhuoya JIA;Yanfang FAN;Yanan Zhao;Xiaohui ZHOU;Kai CAO;Chengde Medical College;

【机构】 Chengde Medical College

【摘要】 [Objectives] To prepare an acute myocardial infarction model for SD rats and conduct ventricular remodeling. [Methods] 45 male Sprague-Dawley( SD) rats were randomly selected in 60 male rats as the model group and the rest rats were sham operation group. For the model group,the left anterior descending coronary artery was ligated,while the sham operation group was not ligated. 4 weeks after the operation,hemodynamics was used to measure the cardiac function of both groups. HE staining and Masson staining were used to observe the changes in myocardial histopathology and myocardial fibrosis of both groups. [Results] Compared with the sham operation group,the model group rats showed significant decrease in the left ventricular systolic pressure( LVSP) and left ventricular pressure maximum rising and dropping rate( ± dp/dtmax) and significant increase in the left ventricular end-diastolic pressure( LVEDP). HE staining showed that a large number of myocardial cell necrosis occured in the model group,while the morphology of myocardial cells of rats in the sham operation group was normal. Masson staining showed that myocardial collagen fibers in the model group were significantly increased compared with those in the sham operation group. Collagen fibers were scattered in the sham operation group. [Conclusions] It can be concluded that cardiac function of rats changed and ventricular remodeling occurred in 4 weeks after myocardial infarction.

【基金】 Supported by Project of Natural Science Foundation of Hebei Province(C2011406009);Key Project of Hebei Provincial Education Department(ZD20131068)
【所属期刊栏目】 Others (2017年06期)
  • 【DOI】10.19600/j.cnki.issn2152-3924.2017.06.018
  • 【分类号】R-332;R542.22
  • 【下载频次】27
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