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Study on Optimization of Culture Conditions for Wild Isaria cicadae Miquel Liquid Strain

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【作者】 Chunqin XIEZheng CAOJunmin FANJunqi XUJun JIA

【Author】 Chunqin XIE;Zheng CAO;Junmin FAN;Junqi XU;Jun JIA;Biological Engineering Department,Jiangsu Polytechnic College of Agriculture and Forestry;

【机构】 Biological Engineering Department,Jiangsu Polytechnic College of Agriculture and Forestry

【摘要】 [Objectives] To study the effect of different culture conditions on the growth of wild I. cicadae Miq. liquid strain. [Methods]The I. cicadae Miq. strain was inoculated into the liquid culture medium with different carbon sources,nitrogen sources,micronutrients and p H,cultured in the constant temperature shaking incubator with rotating speed of 120 r/min at 19℃,and the mycelium pellet diameter,density and weight were compared between different treatments. [Results] The results showed that the optimum components of I. cicadae Miq.strain liquid included soluble starch,milk powder and vitamin B12,and the optimum p H was 5. 0-6. 0. [Conclusions] Soluble starch was the most suitable carbon source for the culture medium of I. cicadae Miq. liquid strain; milk powder was the most suitable nitrogen source for the culture medium of I. cicadae Miq. liquid strain; the most suitable p H was 5. 0-9. 0 for the mycelial growth of I. cicadae Miq.; the formula and mixture ratio of the optimum culture medium for the growth of liquid strain were determined.

【基金】 Supported by"Six Talents Peaks"Project in Jiangsu Province in 2015(NY-024);Project of Jiangsu Polytechnic College of Agriculture and Forestry(2014KJ28)
【所属期刊栏目】 Biotechnology (2018年01期)
  • 【DOI】10.19600/j.cnki.issn2152-3924.2018.01.007
  • 【分类号】S567.3
  • 【下载频次】43
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