节点文献

Molecular Mechanism of Induction on Apoptosis of Human Esophageal Cancer HCE-4 Cells by Active Components from Astragalus membranaceus

免费订阅

【作者】 Jiaru WANGYinghua LUOXianji PIAOChang LIUYi ZHANGHao WANGJinqian LIWanting XUYang LIUYiqin WUChenghao JIN

【Author】 Jiaru WANG;Yinghua LUO;Xianji PIAO;Chang LIU;Yi ZHANG;Hao WANG;Jinqian LI;Wanting XU;Yang LIU;Yiqin WU;Chenghao JIN;College of Life Science & Technology,Heilongjiang Bayi Agricultural University;College of Animal Science & Technology,Heilongjiang Bayi Agricultural University;Department of Gynaecology and Obstetrics,The Fifth Affiliated Hospital of Harbin Medical University;

【机构】 College of Life Science & Technology,Heilongjiang Bayi Agricultural UniversityCollege of Animal Science & Technology,Heilongjiang Bayi Agricultural UniversityDepartment of Gynaecology and Obstetrics,The Fifth Affiliated Hospital of Harbin Medical University

【摘要】 [Objectives] To investigate the pharmacologic effects of active components from A. membranaceus on human esophageal cancer HCE-4 cells and its apoptosis mechanism. [Methods] The viabilities of HCE-4 cells were measured by MTT assay. The induction of active components from A. membranaceus on apoptosis of HCE-4 cells was detected by Annexin V-FITC/PI double staining. The apoptotic-related protein expression levels were determined by Western blotting. [Results] Formononetin and astragaloside IV suppressed the proliferation of HCE-4 cells in a dose-dependent manner. The Annexin V-FITC/PI double staining results showed that formononetin and astragaloside IV could induce HCE-4 cells apoptosis in a time-dependent manner. The Western blotting results showed that formononetin and astragaloside IV could significantly down-regulate p-AKT,pro-caspase-3,and increase cle-caspase-3 protein expression in HCE-4 cells. [Conclusions]Active components from A. membranaceus such as formononetin and astragaloside IV significantly inhibited the proliferation of human esophageal cancer HCE-4 cells by inducing mitochondrial dependent apoptosis via AKT signaling pathway.

【基金】 Supported by the Nature Science Foundation of Heilongjiang Province of China(LC2015036);the Program of Cultivation and Support Projects of Heilongjiang Bayi Agricultural University(XA2015-04);the Research Project of Heilongjiang Bayi Agricultural University(XYB2013-24);the Postdoctoral Scientific Research Foundation of Heilongjiang Province of China(LBH-Q13132);the Scientific Research Innovation Program for College Graduates of Heilongjiang Bayi Agricultural University(YJSCX2017-Y72)
【所属期刊栏目】 Clinical Application (2018年01期)
  • 【DOI】10.19600/j.cnki.issn2152-3924.2018.01.016
  • 【分类号】R285
  • 【下载频次】33
节点文献中: 

本文链接的文献网络图示:

浏览历史:
下载历史: