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Alcohols Extraction Process and Content of Active Ingredients in Chinese Northeastern Auricularia auricula

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【作者】 Xianghui KONGGuoli YANGXijun CHENHui WANGDan CHEN

【Author】 Xianghui KONG;Guoli YANG;Xijun CHEN;Hui WANG;Dan CHEN;Institute of Microbiology,Heilongjiang Academy of Sciences;Institute of Advanced Technology,Heilongjiang Academy of Sciences;Heilongjiang Engineering Technology Research Center of Black Fungus Resource Utilization;Heilongjiang Johnsun Biological Engineering Co.,Ltd.;Heilongjiang Engineering Technology Research Center of Mycomedicine;

【通讯作者】 Xijun CHEN;

【机构】 Institute of Microbiology,Heilongjiang Academy of SciencesInstitute of Advanced Technology,Heilongjiang Academy of SciencesHeilongjiang Engineering Technology Research Center of Black Fungus Resource UtilizationHeilongjiang Johnsun Biological Engineering Co.,Ltd.Heilongjiang Engineering Technology Research Center of Mycomedicine

【摘要】 [Objectives] The aim was to study the type and content of alcohol-soluble active ingredients in Auricularia auricula. [Methods]With total yield of alcohol extract as an indicator,the optimal concentrations of ethanol,isopropanol and n-butanol for alcohol extraction of A. auricula powder were determined. The contents of total flavonoids,polyphenols and triterpenoids in three different alcohol extracts of A. auricula,as well as in the alcohol extracts of polysaccharides and melanin extraction residue of A. auricula were determined. [Results] When90% ethanol was used,the yield of alcohol extract of A. auricula was 10. 7%. In the alcohol extract,the contents of total flavonoids,polyphenols and triterpenoids were 5. 68 mg/100 g,0. 43% and 0. 55%,respectively. When 100% isopropanol was used,the yield of alcohol extract was 10. 1%. In the alcohol extract,the contents of total flavonoids,polyphenols and triterpenoids were 4. 84 mg/100 g,0. 66% and 0. 37%,respectively. When 70% n-butanol was used,the yield of alcohol extract was 5. 4%. In the alcohol extract,the contents of total flavonoids,polyphenols and triterpenoids were 4. 07 mg/100 g,1. 68% and 0. 30%,respectively. The yields of ethanol,isopropanol and n-butanol extracts of polysaccharide and melanin extraction residue of A. auricula were 13. 8%,14. 3% and 8. 9%,respectively. In the three kinds of alcohol extracts,the contents of total flavonoids were 8. 28,11. 99,and 12. 38 mg/100 g,respectively; the contents of polyphenols were 0.14%,0. 38% and 0. 75%,respectively; and the contents of triterpenoids were 0. 25%,0. 60% and 0. 58%,respectively. [Conclusions]This study will provide certain reference for the in-depth study and development and utilization of active ingredients in A. auricula.

【基金】 Supported by Special Funds of Innovation Ability Promotion of Heilongjiang Scientific Research Institution(YC2015D002);Scientific Research Foundation of Heilongjiang Academy of Sciences(KY2018SW02);Funds for Harbin Applied Technology Research and Development(2016RAXYJ046)
【所属期刊栏目】 Pharmacological and Toxicological Analyses (2018年04期)
  • 【DOI】10.19600/j.cnki.issn2152-3924.2018.04.012
  • 【分类号】R284.2
  • 【被引频次】1
  • 【下载频次】27
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