节点文献

Extraction Process and Content Determination of Caffeic Acid in Laggera alata from Different Production Areas of Guangxi

免费订阅

【作者】 Jiangcun WEIYong CHENZujie QINJiabao MAShengbin CHENZuliang QUEJinzhou LIZijun CHEN

【Author】 Jiangcun WEI;Yong CHEN;Zujie QIN;Jiabao MA;Shengbin CHEN;Zuliang QUE;Jinzhou LI;Zijun CHEN;Guangxi Zhuang International Hospital;Guangxi University of Chinese Medicine;The First Affiliated Hospital of Guangxi University of Chinese Medicine;

【通讯作者】 Yong CHEN;

【机构】 Guangxi Zhuang International HospitalGuangxi University of Chinese MedicineThe First Affiliated Hospital of Guangxi University of Chinese Medicine

【摘要】 [Objectives] To establish a method for determining the content of Laggera alata( D. Don) Sch. Bip. Ex Oliv. using caffeic acid the target component,and to compare the content of caffeic acid in the medicinal materials of L. alata in different production areas of Guangxi.[Methods]The content was determined by InertsilODS-3 chromatographic column C18( 4. 60 mm × 250 mm,5 μm,mobile phase: acetonitrile-0. 1% phosphoric acid( 22∶ 78),detection wavelength: 320 nm,flow rate: 1. 0 m L/min,column temperature: 30℃,and injection volume: 10 μL. [Results] The caffeic acid showed a good linear relationship in the range of injection volume of 0. 025 92-0. 259 2 μg( R =0. 999 5). The average recovery rate was 98. 33%( RSD = 1. 85%). L. alata in different production areas of Guangxi contained the caffeic acid,and there was a great difference in the caffeic acid. L. alata in Baise had the highest content of caffeic acid,while that in Guilin had the lowest content of caffeic acid. [Conclusions]This method can accurately determine the content of caffeic acid and is expected provide a scientific basis for the development and utilization of herbal medicine L. alata.

【基金】 Supported by Project of National Natural Science Foundation(81660701&81260673);Project of Guangxi Graduate Education Innovation(YJS201625);Natural Science Foundation Project of Guangxi(2016GXNSFAA380148&2014GXNSFAA118208);Program of Key Laboratory for Purification and Quality Analysis of TCM Extraction in Guangxi Universities(Gui Jiao Ke Yan[2014]No.6);Laboratory of Chemistry and Quality Analysis in the Third Level Laboratory for Research of TCM(Zhuang)of State Administration of Traditional Chinese Medicine(Guo Zhong Yi Yao Fa[200]No.21)
【所属期刊栏目】 Processing of Traditional Chinese Medicine and Quality Control (2018年04期)
  • 【DOI】10.19600/j.cnki.issn2152-3924.2018.04.021
  • 【分类号】R284
  • 【被引频次】1
  • 【下载频次】16
节点文献中: 

本文链接的文献网络图示:

浏览历史:
下载历史: