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Apoptotic Effect of Cryptotanshinone on Human Melanoma A375 Cells

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【作者】 Jinqian LIJiaru WANGLingqi MENGYi ZHANGHao WANGWanting XUYang LIUYu ZHANGShinong WANGYuqing ZHAIYinghua LUOChenghao JIN

【Author】 Jinqian LI;Jiaru WANG;Lingqi MENG;Yi ZHANG;Hao WANG;Wanting XU;Yang LIU;Yu ZHANG;Shinong WANG;Yuqing ZHAI;Yinghua LUO;Chenghao JIN;College of Life Science & Technology,Heilongjiang Bayi Agricultural University;

【通讯作者】 Chenghao JIN;

【机构】 College of Life Science & Technology,Heilongjiang Bayi Agricultural University

【摘要】 [Objectives] The aim was to investigate the effect of cryptotanshinone on apoptosis of human melanoma A375 cells and its related mechanism of mitochondrial pathway.[Methods]The cytotoxic effect of cryptotanshinone on apoptosis of human melanoma A375 cells was detected by MTT colorimetry.The apoptosis of melanoma A375 cells was detected with Annexin V-FITC/PI and observed by fluorescence inverted microscope.The expression of apoptosis-related proteins was detected by Western blotting.[Results]The viability of the A375 cells decreased with the increase of drug concentration.The fluorescence intensity of the cells increased with the treatment time.The expression of pro-apoptotic protein caspase-3 gradually increased,while the expression of apoptosis-inhibiting proteins p-AKT and Bcl-2 gradually reduced.[Conclusions]Cryptotanshinone induces apoptosis of human melanoma A375 cells via AKT signaling pathway,thus exerting a good cytotoxic effect on A375 cells.

【基金】 Supported by Multigrain Production and Processing Characteristic Discipline Construction Project;Postdoctoral Scientific Research Foundation of Heilongjiang Province of China(LBH-Q13132)
【所属期刊栏目】 Pharmacological and Toxicological Analyses (2018年06期)
  • 【DOI】10.19600/j.cnki.issn2152-3924.2018.06.010
  • 【分类号】R285.5
  • 【下载频次】24
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