节点文献

Screening of Anti-tumor Effective Extracts from Sedum bulbiferum Makino and HPLC Determination of Quercetin and Kaempferol in This Herbal Medicine

免费订阅

【作者】 Liming MENGXiangluan WANLuyang LIJing HUPu CHENDingrong WAN

【Author】 Liming MENG;Xiangluan WAN;Luyang LI;Jing HU;Pu CHEN;Dingrong WAN;Shiyan Institute for Food and Drug Control;School of Foreign Languages,Hubei University of Chinese Medicine;College of Pharmacy,South-central University for Nationalities;

【通讯作者】 Dingrong WAN;

【机构】 Shiyan Institute for Food and Drug ControlSchool of Foreign Languages,Hubei University of Chinese MedicineCollege of Pharmacy,South-central University for Nationalities

【摘要】 [Objectives] To investigate the anti-tumor activity of Sedum bulbiferum Makino in vitro,and establish a HPLC method for determination of quercetin and kaempferol in S. bulbiferum. [Methods] The inhibitory activities of different extracts and total flavonoids of S. bulbiferum on proliferation of three kinds of cancer cells( Hep G2,EC109,SW480) were tested by MTT assay. HPLC method for determination of quercetin and kaempferol in S. bulbiferum was established. [Results]The growth and proliferation of the three kinds of cancer cells were all significantly inhibited by ethyl acetate fraction and total flavonoids isolated from S. bulbiferum. With each extraction concentration increasing,stronger anti-tumor activity was found. The linear ranges of quercetin and kaempferol were 0. 03-0. 36 μg( R = 0. 999 9) and0. 08-0. 96 μg( R = 0. 999 9),and the average recoveries were 98. 90%( RSD = 1. 15%) and 98. 27%( RSD = 1. 70%),respectively.[Conclusions]S. bulbiferum has significant anti-tumor activity in vitro. The HPLC method established was accurate,reproducible,and could be used for quality control of this crude drug.

【所属期刊栏目】 Chemical Composition of Natural Medicine (2019年03期)
  • 【DOI】10.19600/j.cnki.issn2152-3924.2019.03.007
  • 【分类号】R284;O657.72
  • 【下载频次】17
节点文献中: 

本文链接的文献网络图示:

浏览历史:
下载历史: