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Simultaneous Determination of Four Amino Acids in Fufang Ejiao Buxue Granule by HPLC

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【作者】 Ningning YINDongxiao GUO

【Author】 Ningning YIN;Dongxiao GUO;Shandong Center for Food and Drug Evaluation & Certification;Shandong Institute for Food and Drug Control;

【通讯作者】 Dongxiao GUO;

【机构】 Shandong Center for Food and Drug Evaluation & CertificationShandong Institute for Food and Drug Control

【摘要】 [Objectives] To establish an High Performance Liquid Chromatography(HPLC) method for simultaneous determination of four amino acids in Fufang Ejiao Buxue Granule. [Methods] The quantitative analysis was carried out with a Waters Sunfire C18 column(4.6 mm × 250 mm, 5 μm). A binary mobile solvent was used: mobile solvent A was acetonitrile-0.1 mol/L sodium acetate solution(adjusting pH to 6.5 with 36% acetic acid)(7∶93) and mobile solvent B was acetonitrile-H2O(4∶1). The mobile phase was delivered at a flow rate of 1.0 mL/min with a gradient elution profile(0-13 min, 100% A → 93% A; 13-17.9 min, 93% A → 88% A; 17.9-29.0 min, 88% A → 85% A; 29-39 min, 85% A → 66% A; 39-45 min, 66% A → 0% A). The column temperature was at 43 ℃. The detection wavelength was 254 nm. [Results] The injection volume of L-hydroxyproline, glycine, alanine, and L-proline showed a good linear relationship with the chromatographic peak area in the range of 0.012 to 0.117, 0.022 to 0.218, 0.010 to 0.097, 0.016 to 0.160 μg, separately. The average recovery rate(n=6) was 96.4%, 97.3%, 97.1%, and 99.4%, respectively; the relative standard deviations were 1.2%, 1.9%, 1.7%, and 0.9%, respectively. [Conclusions] This method is simple in operation and good in reproducibility, and provides a reliable method for controlling the quality of Fufang Ejiao Buxue Granules.

【所属期刊栏目】 Chemical Composition of Natural Medicine (2019年05期)
  • 【DOI】10.19600/j.cnki.issn2152-3924.2019.05.006
  • 【分类号】R286.0;O657.72
  • 【下载频次】10
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