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Optimization of Extraction Process of Total Flavonoids and Chlorogenic Acid from Flos Lonicerae by Orthogonal Experiment

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【作者】 Huasheng LIZhenhua ZHOUShouyong TANG

【Author】 Huasheng LI;Zhenhua ZHOU;Shouyong TANG;Yongzhou Vocational and Technical College;

【通讯作者】 Huasheng LI;

【机构】 Yongzhou Vocational and Technical College

【摘要】 [Objectives] To explore the process of simultaneous extracting total flavonoids and chlorogenic acid from Flos Lonicerae by pressure assisted extraction. [Methods] Based on the single factor experiment, orthogonal experiments were carried out to investigate the effects of solvent concentration, solid-to-liquid ratio, extraction pressure, extraction time and extraction temperature on the extraction rate of total flavonoids and chlorogenic acid. [Results] The results showed that the extraction pressure had a significant effect on the extraction of two kinds of chemical constituents from F. Lonicerae. The optimum process conditions were as follows: solvent concentration was 60%, solid-liquid ratio was 1∶20(g/mL), extraction pressure was 2 MPa, extraction time was 30 min, extraction temperature was 70 ℃. The process conditions are stable, and the extraction efficiency is higher than that of ultrasonic assisted extraction method. Under the test conditions, the extraction rates of total flavonoids and chlorogenic acids were respectively 15.66% and 3.89%. [Conclusions] This study provides a new theoretical basis for the development and utilization of F. Lonicerae.

【基金】 Supported by Scientific Research Project of Hunan Provincial Department of Education in 2017(17C1603)
【所属期刊栏目】 Processing of Traditional Chinese Medicine and Quality Control (2019年05期)
  • 【DOI】10.19600/j.cnki.issn2152-3924.2019.05.016
  • 【分类号】R284.2
  • 【下载频次】20
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