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Determination of Naringin Content in Peel of Guangxi Citrus maxima (Burm.) Merr by HPLC

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【作者】 Chenyan LIANGLu ZHANGCuiliu PANYunli TANGRongling MA

【Author】 Lu ZHANG;Cuiliu PAN;Yunli TANG;Rongling MA;Guangxi University of Chinese Medicine;Liuzhou Worker’s Hospital;

【通讯作者】 Yunli TANG;Rongling MA;

【机构】 Guangxi University of Chinese MedicineLiuzhou Worker’s Hospital

【摘要】 [Objectives] To establish a method for determining the naringin content in the peel of Guangxi Citrus maxima(Burm.) Merr. [Methods] The high performance liquid chromatography(HPLC) method was applied. The chromatographic column was Agilent HC-C18(4.6 mm×250 mm, 5 μm); the mobile phase was acetonitrile-0.1% phosphoric acid(18∶82); the flow rate was 1.0 mL/min; the column temperature was 25 ℃; the detection wavelength was 283 nm. [Results] Naringin showed a good linear relationship in the range of 0.164-3.27 μg, r=0.999 9. The average recovery rate was 98.66%, and RSD=1.80%(n=6). [Conclusions] This method is simple, feasible, reproducible, and accurate, so it can be used for the determination of naringin content in the peel of Guangxi C. maxima.

【基金】 Supported by Project for Improving Basic Research Ability of Middle Aged and Young Teachers in Colleges and Universities of Guangxi in 2019(2019KY0324);Program of Collaborative Innovation Center of Zhuang and Yao Medicine(zyx[2015]-01);Project of Guangxi University of Chinese Medicine(P2010016)
【所属期刊栏目】 Chemical Composition of Natural Medicine (2019年06期)
  • 【DOI】10.19600/j.cnki.issn2152-3924.2019.06.009
  • 【分类号】R284.1
  • 【下载频次】1
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