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Inhibitiont of Obazema on Proliferation of Gastric Cancer BGC-823 Cells and Its Mechanism

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【作者】 Ying LIRisha WEIZEHairong ZHONGJixiu SHENYisong LIYuan LIU

【Author】 Ying LI;Risha WEIZE;Hairong ZHONG;Jixiu SHEN;Yisong LI;Yuan LIU;College of Pharmacy, Southwest Minzu University;Ethnic Medicine Institute, Southwest Minzu University;

【通讯作者】 Yuan LIU;

【机构】 College of Pharmacy, Southwest Minzu UniversityEthnic Medicine Institute, Southwest Minzu University

【摘要】 [Objectives] This study aimed to investigate the inhibiting effect of Obazema on proliferation of gastric cancer BGC-823 cells and its mechanism. [Methods] BGC-823 cells were treated with high, medium and low concentrations of drug-containing serum(0.316%, 0.158% and 0.079%) for 0, 48, 72 and 96 h, respectively. Then, the proliferation of the cells was detected with CCK-8 method, and the expression of related proteins, B lymphocytoma-2(Bcl-2), phosphorylated protein kinase B(p-Akt), protein kinase B(Akt) and glyceraldehyde-3-phosphate dehydrogenase(GAPDH), was detected using Western blotting. [Results] The proliferation of the BGC-823 cells was significantly inhibited with different doses of Boenninghausenia albiflora(Hook.) Reichb. ex Meisn. var. albiflora(CH) and B. sessilicarpa Lévl.(S)(P<0.01), in dose-dependent and time-dependent manners. The inhibition of high-dose S on cell proliferation was similar to that of CTX 48 h after administration; the inhibition of high-dose CH on cell proliferation was significantly stronger than that of CTX(P<0.01); different doses of drug administration groups significantly inhibited the expression of p-Akt and Bcl-2 in the BGC-823 cells; the inhibition of high-dose CH on the expression of P-Akt and Bcl-2 and the inhibition of medium-dose CH on the expression of Bcl-2 were significantly stronger than that of CTX(P<0.05, P<0.01), in a certain dose-dependent manner; at the same dose, the inhibition of CH on the expression of the proteins was stronger than that of S(P<0.05, P<0.01); administration of S and CH significantly inhibited the expression of GAPDH compared with CTX(P<0.05, P<0.01). [Conclusions] Obazema has the capacity to inhibit the proliferation of BGC-823 cells. The mechanism may be achieved by inhibiting the expression of p-Akt and Bcl-2, and GAPDH may be the target gene of its anti-tumor mechanism. The inhibiting effect of CH on BGC-823 cells was more significantly than that of S.

【基金】 Supported by National Key Technology Research and Development Program(2018FYC1708000);Basic Research Project for Application of Science and Technology in Sichuan Province(2017JY0274);Science and Technology Research Project of Sichuan Traditional Chinese Medicine Administration(2018JC028)
【所属期刊栏目】 Pharmacological and Toxicological Analyses (2019年06期)
  • 【DOI】10.19600/j.cnki.issn2152-3924.2019.06.013
  • 【分类号】R285
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